Characterization of bioactive compounds and ameliorative effects of Ceratonia siliqua leaf extract against CCl4 induced hepatic oxidative damage and renal failure in rats

被引：65

作者：

Ben Hsouna, Anis ^{[2
]}

Saoudi, Mongi ^{[1
]}

Trigui, Mohamed ^{[2
]}

Jamoussi, Kamel ^{[3
]}

Boudawara, Tahia ^{[4
]}

Jaoua, Samir ^{[2
,5
]}

El Feki, Abdelfattah ^{[1
]}

机构：

[1] Sci Fac Sfax, Dept Life Sci, Anim Ecophysiol Lab, Sfax 3000, Tunisia

[2] Ctr Biotechnol Sfax, Biopesticides Team LPIP, Sfax 3018, Tunisia

[3] CHU Habib Bourguiba Sfax, Biochem Lab, Sfax, Tunisia

[4] CHU Habib Bourguiba Sfax, Histopathol Lab, Sfax, Tunisia

[5] Qatar Univ, Coll Arts & Sci, Doha, Qatar

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2011年 / 49卷 / 12期

关键词：

Ceratonia siliqua; Carbon tetrachloride; Oxidative stress; Protective effect; Phenolic compounds; ANTIOXIDANT ACTIVITY; CARBON-TETRACHLORIDE; PROTECTIVE ROLE; FREE-RADICALS; VITAMIN-E; STRESS; POLYPHENOLS; DIETARY; NEPHROTOXICITY; OREGANO;

D O I：

10.1016/j.fct.2011.09.034

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Ceratonia siliqua is a typical Mediterranean plant, mainly used in food and Tunisian traditional folk medicine. Among the tested extracts, the ethyl acetate fraction (EACs) exhibited the highest total phenolic and flavonoids content. The antioxidant activity in vitro systems showed a more significant potent free radical scavenging activity of this extract than other analysis fractions. The HPLC finger print of EACs active extract showed the presence of six phenolic compounds. The in vivo results showed that oral administration of CCl4 enhanced levels of hepatic and renal markers (ALT, AST, ALP, LDH, gamma-GT, urea and creatinine) in the serum of experimental animals. It also increased the oxidative stress markers resulting in increased levels of the lipid peroxidation with a concomitant decrease in the levels of enzymatic antioxidants (SOD, CAT, GPx) in both liver and kidney. The pre-treatment of experimental rats with 250 mg/kg (BW) of the EACs, by intraperitoneal injection for 8 days, prevented CCl4 induced disorders in the levels of hepatic and kidney markers. The biochemical changes were in accordance with histopathological observations suggesting a marked hepatoprotective and nephroprotective effect of the EACs extract. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：3183 / 3191

页数：9

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