Proteomics Analysis of Porcine Serum Proteins by LC-MS/MS after Foot-and-Mouth Disease Virus (FMDV) Infection

被引：7
|
作者：
Liu, Yongjie [1 ]
Zhang, Keshan [1 ]
Zheng, Haixue [1 ]
Shang, Youjun [1 ]
Guo, Jianhong [1 ]
Tian, Hong [1 ]
Lu, Guodong [1 ]
Jin, Ye [1 ]
He, Jijun [1 ]
Cai, Xuepeng [1 ]
Li, Xiangtao [1 ]
机构：
[1] Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Natl Food & Mouth Dis Reference Lab, Lanzhou 730046, Gansu, Peoples R China
来源：
JOURNAL OF VETERINARY MEDICAL SCIENCE | 2011年 / 73卷 / 12期
关键词：
foot and mouth disease virus infection; liquid chromatography-tandem mass spectrometry; porcine serum; proteomics analysis; MASS-SPECTROMETRY; PLASMA; CELLS; EXPRESSION;
D O I：
10.1292/jvms.11-0019
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
To analyze serum proteomics differences between normal and foot and mouth disease virus (FMDV)-infected piglets, an analytical method based on liquid chromatography with tandem mass spectrometry (LC-MS/MS) was used. Samples of venous blood were collected before and after FMDV infection and high abundance serum albumin was removed using a commercial kit. After trypsin digestion, serum samples were processed with LC-MS/MS. Proteins were identified by peptide mass fingerprinting. We found that apolipoprotein A-IV precursor, haptoglobin and probable chemoreceptor glutamine deamidase cheD appeared after FMDV infection in the same piglet. This is believed to be the first time that serum proteomics analysis by LC-MS/MS after FMDV infection has been performed, and our results may provide further information about biomarkers for early diagnosis of FMD in piglets.
引用
收藏
页码：1569 / 1572
页数：4
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