Comparative genomics analysis of Acinetobacter haemolyticus isolates from sputum samples of respiratory patients

被引：14

作者：

Bai, Liang ^{[1
]}

Zhang, ShaoCun ^{[1
]}

Deng, Yong ^{[1
]}

Song, ChengCheng ^{[2
]}

Kang, GuangBo ^{[1
]}

Dong, Yan ^{[2
]}

Wang, Yue ^{[3
]}

Gao, Feng ^{[4
]}

Huang, He ^{[1
]}

机构：

[1] Tianjin Univ, Sch Chem Engn & Technol, Dept Biochem Engn, Minist Educ,Key Lab Syst Bioengn, Tianjin 300072, Peoples R China

[2] BGI TJ, Tianjin 300308, Peoples R China

[3] Tianjin Med Univ, Inst Infect Dis, Hosp 2, Tianjin 300211, Peoples R China

[4] Tianjin Univ, Frontier Sci Ctr Synthet Biol MOE, Collaborat Innovat Ctr Chem Sci & Engn, Dept Phys,Sch Sci,Key Lab Syst Bioengn MOE, Tianjin 300072, Peoples R China

来源：

GENOMICS | 2020年 / 112卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Acinetobacter haemolyticus; Comparative genomics; Pathogenicity; Resistance; Plasmid-mediated; WEB-BASED TOOL; DNA METHYLATION; BAUMANNII; ANNOTATION; RESISTANCE; GENES; SEQUENCE; DATABASE; PLASMID; EXPRESSION;

D O I：

10.1016/j.ygeno.2020.03.016

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Acinetobacter haemolyticus (A. haemolyticus) is a significant Acinetobacter pathogen, and the resistance of A. haemolyticus continues to rise due to abuse of antibiotics and the frequent gene exchange between bacteria in hospital. In this study, we performed complete genome sequencing of two A. haemolyticus strains TJR01 and TJS01 to improve our understanding of pathogenic and resistance of A. haemolyticus. Both TJR01 and TJS01 contain one chromosome and two plasmids. Compared to TJS01, more virulence factors (VFs) associated pathogenicity and resistant genes were predicted in TJR01 due to T4SS and integron associated with combination and transport. Antimicrobial susceptibility results were consistent with sequencing. We suppose TJS01 was a susceptive strain and TJR01 was an acquired multidrug resistance strain due to plasmid-mediated horizontal gene transfer. We hope these findings may be helpful for clinical treatment of A. haemolyticus infection and reduce the risk of potential outbreak infection.

引用

页码：2784 / 2793

页数：10

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