CircUBE2Q2 promotes differentiation of cattle muscle stem cells and is a potential regulatory molecule of skeletal muscle development

被引:15
作者
Zhang, Rui-Men [1 ]
Pan, Yu [1 ]
Zou, Chao-Xia [1 ]
An, Qiang [1 ]
Cheng, Juan-Ru [1 ]
Li, Peng-Ju [1 ]
Zheng, Zi-Hua [1 ]
Pan, Yan [2 ]
Feng, Wan-You [1 ]
Yang, Su-Fang [1 ,3 ]
Shi, De-Shun [1 ]
Wei, Ying-Ming [1 ]
Deng, Yan-Fei [1 ]
机构
[1] Guangxi Univ, State Key Lab Conservat & Utilizat Subtrop Agrobi, Anim Reprod Inst, Nanning 530004, Peoples R China
[2] Guangxi Agr Vocat Univ, Nanning 530007, Guangxi, Peoples R China
[3] Guangxi Univ Chinese Med, Int Zhuang Med Hosp, Nanning 530000, Guangxi, Peoples R China
基金
中国国家自然科学基金;
关键词
Cattle; Muscle stem cells; circRNAs; Proliferation; Differentiation; CIRCULAR RNA; NONCODING RNAS; PROLIFERATION; MYOBLASTS; INHIBITION; WIDESPREAD; MYOGENESIS; GROWTH;
D O I
10.1186/s12864-022-08518-4
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background The growth and development of muscle stem cells (MuSCs) are significant events known to affect muscle plasticity, disease, meat production, and meat quality, which involves the types and functions of mRNA and non-coding RNA. Here, MuSCs were cultured from Guangxi fetal cattle. RNA sequencing was used to analyze the RNA expression of mRNA and non-coding RNAs during the cell proliferation and differentiation phases. Results Two thousand one hundred forty-eight mRNAs and 888 non-coding RNAs were differentially expressed between cell proliferation and differentiation phases, including 113 miRNAs, 662 lncRNAs, and 113 circRNAs. RT-qPCR verified the differential expression levels of mRNAs and non-coding RNAs, and the differentially expressed circUBE2Q2 was subsequently characterized. Expression profile analysis revealed that circUBE2Q2 was abundant in muscle tissues and intramuscular fat. The expression of cricUBE2Q2 was also significantly upregulated during MuSCs myogenic differentiation and SVFs adipogenic differentiation and decreased with age in cattle muscle tissue. Finally, the molecular mechanism of circUBE2Q2 regulating MuSCs function that affects skeletal muscle development was investigated. The results showed that circUBE2Q2 could serve as a sponge for miR-133a, significantly promoting differentiation and apoptosis of cultured MuSCs, and inhibiting proliferation of MuSCs. Conclusions CircUBE2Q2 is associated with muscle growth and development and induces MuSCs myogenic differentiation through sponging miR-133a. This study will provide new clues for the mechanisms by which mRNAs and non-coding RNAs regulate skeletal muscle growth and development, affecting muscle quality and diseases.
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页数:16
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