Involvement of the PLCε/PKCα pathway in human BIU-87 bladder cancer cell proliferation

被引：25

作者：

Yan Ling ^{[1
]}

Luo Chunli ^{[1
]}

Wu Xiaohou ^{[2
]}

Zhang Qiaoling ^{[1
]}

机构：

[1] Chongqing Med Univ, Dept Lab Med Diagnost, Chongqing 400016, Peoples R China

[2] Chongqing Med Univ, Affiliated Hosp 1, Dept Urinary Surg, Chongqing 400016, Peoples R China

来源：

CELL BIOLOGY INTERNATIONAL | 2011年 / 35卷 / 10期

关键词：

bladder cancer; phospholipase C epsilon; proliferation; protein kinase C alpha; small-hairpin RNA (shRNA); PROTEIN-KINASE-C; CYCLIN D1; EXPRESSION; GROWTH; CARCINOMA; JUN;

D O I：

10.1042/CBI20090101

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

PLC epsilon (phospholipase C epsilon), one of effectors belonging to the small GTPase superfamily, has been suggested to play a crucial role in carcinogenesis. However, its bio-function in bladder cancer has never been demonstrated. In our previous study, we found that PLC epsilon mRNA was highly expressed in bladder cancer tissues. In the present study, we silenced the PLC epsilon gene by shRNA (small-hairpin RNA) in the bladder cancer cell line BIU-87. The results showed that it significantly inhibited cell proliferation and arrested the cell cycle at G(0)/G(1)-phase. The regulation of cell characteristics has been related to PKC alpha (protein kinase C alpha) activity. Further study showed that knockdown of the PLC epsilon gene down-regulated oncogenes c-fos and c-jun. These results indicate that PLC epsilon plays a crucial role in bladder cancer, and PLC epsilon may be a key molecule regulating the signal pathway of bladder cancer proliferation.

引用

页码：1031 / 1036

页数：6

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