SAG2 locus genotyping of Toxoplasma gondii in meat products of East Azerbaijan Province, North West of Iran During 2010-2011

被引：0

作者：

Fallah, Esmaeil ^{[1
]}

Hajizadeh, Maryam ^{[1
]}

Farajnia, Safar ^{[2
]}

Khanmahammadi, Majid ^{[3
]}

机构：

[1] Tabriz Univ Med Sci, Dept Parasitol, Fac Med, Tabriz, Iran

[2] Tabriz Univ Med Sci, Dept Biotechnol, Drug Appl Res Ctr, Tabriz, Iran

[3] Islamic Azad Univ, Marand Branch, Dept Lab Sci, Marand, Iran

来源：

AFRICAN JOURNAL OF BIOTECHNOLOGY | 2011年 / 10卷 / 62期

关键词：

Toxoplasma gondii; SAG2; genotyping; meat products; STRAINS; PREVALENCE; INFECTION; CHICKENS; ANIMALS;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Toxoplasmosis is an infection caused by Toxoplasma gondii, an intracellular obligate parasite. Its transmission is usually attributed to ingestion of undercooked or raw meat. The aim of this study was the detection and genotyping of T. gondii in meat products using the molecular method in East Azerbaijan. DNA was extracted from 164 meat products sample obtained from 15 commercial establishments. Nested polymerase chain reaction with specific primers for Toxoplasma gondii SAG2 locus was used for detection of the parasite in samples. Genotyping was carried out by digestion of PCR products with restriction enzymes Cfo1 and Sau3AI. T. gondii DNA was detected in 16.6, 19.1, 15 and 56.6%, in salami, sausages, and hamburger and kebab samples, respectively. Genotyping by restriction fragment length polymorphism (RFLP) analysis of SAG2 locus revealed that all of the samples belonged to genotype I. The detection of the parasite in uncooked meat and commercial meat products, and the high ratio of seropositive slaughtered animals, emphasis that the risk still exists for food -born toxoplasmosis.

引用

页码：13631 / 13635

页数：5

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