YY1-induced lncRNA ZFPM2-AS1 facilitates cell proliferation and invasion in small cell lung cancer via upregulating of TRAF4

被引：44
作者：
Yan, Zhijun [1 ]
Yang, Qilian [2 ]
Xue, Min [1 ]
Wang, Sheng [3 ]
Hong, Weijun [1 ]
Gao, Xiwen [1 ]
机构：
[1] Fudan Univ, Minhang Hosp, Dept Resp Med, 170 Xinsong Rd, Shanghai 201199, Peoples R China
[2] Fudan Univ, Minhang Hosp, Dept Pharm, 170 Xinsong Rd, Shanghai 201199, Peoples R China
[3] Fudan Univ, Inst Biomed Sci, 131 Dongan Rd, Shanghai 200032, Peoples R China
来源：
CANCER CELL INTERNATIONAL | 2020年 / 20卷 / 01期
关键词：
ZFPM2-AS1; miR-3612; TRAF4; YY1; SCLC; COMPETING ENDOGENOUS RNA; EPITHELIAL-MESENCHYMAL TRANSITION; LONG NONCODING RNAS; PROMOTES; GROWTH; CERNA; PROGRESSION; OVEREXPRESSION; TUMORIGENESIS; METASTASIS;
D O I：
10.1186/s12935-020-1157-7
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Background Newly identified lncRNA zinc finger protein, FOG family member 2 antisense RNA 1 (ZFPM2-AS1) is identified as an oncogenic gene. However, the role of ZFPM2-AS1 in small cell lung cancer (SCLC) is poorly comprehended. Methods The expression of genes in SCLC tissues and cells was measured by qRT-PCR. Colony formation, EdU, CCK-8, transwell and wound healing as well as in vivo assays revealed the function of ZFPM2-AS1 in SCLC. ChIP, luciferase reporter, RIP and RNA pull down assays demonstrated the binding relation among genes. Results ZFPM2-AS1 was significantly upregulated in SCLC tissues and cells. ZFPM2-AS1 deficiency attenuated SCLC cell proliferation, invasion and migration. In addition, ZFPM2-AS1 was transcriptionally activated by Yin Yang 1 (YY1) factor. Further, miR-3612 was confirmed as downstream miRNA of ZFPM2-AS1. Moreover, TNF receptor associated factor 4 (TRAF4) was the target gene of miR-3612 in SCLC. ZFPM2-AS1, miR-3612 and TRAF4 jointly constituted a competing endogenous RNA (ceRNA) network in SCLC. Finally, TRAF4 could countervail ZFPM2-AS1 downregulation-mediated function on SCLC cell proliferation and invasion in vitro and tumor growth in vivo. Conclusion Our study elucidated the oncogenic effect of ZFPM2-AS1 in SCLC progression, indicating it may be a therapeutic target for SCLC.
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页数：11
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