Cercosporin production by Cercospora coffeicola isolates: spectrophotometry and HPLC quantification and image analysis

被引：2

作者：

dos Santos Botelho, Deila Magna ^{[1
]}

Vilela de Resende, Mario Lucio ^{[1
]}

Teixeira, Alexandre Rezende ^{[1
]}

Santiago, Wilder Douglas ^{[2
]}

Pozza, Edson Ampelio ^{[1
]}

Moreira, Silvino Intra ^{[1
]}

Ramos, Juliana Barros ^{[1
]}

de Aquino, Sinara Oliveira ^{[2
]}

Ferreira, Antonio Gilberto ^{[3
]}

Reichel, Tharyn ^{[1
]}

Guerra-Guimaraes, Leonor ^{[4
,5
]}

机构：

[1] Univ Fed Lavras, Dept Fitopatol, Caixa Postal 3037, Lavras, MG, Brazil

[2] Univ Fed Lavras, Dept Quim, Caixa Postal 3037, Lavras, MG, Brazil

[3] Univ Fed Sao Carlos, Dept Quim, Sao Carlos, SP, Brazil

[4] Univ Lisbon, Ctr Invest Ferrugens Cafeeiro, Inst Super Agron, Lisbon, Portugal

[5] Univ Lisbon, Inst Super Agron, Linking Landscape Environm Agr & Food, Lisbon, Portugal

来源：

CHEMICAL PAPERS | 2022年 / 76卷 / 04期

关键词：

Microscopy; Toxin; High-performance liquid chromatography; Methods; TEMPERATURE;

D O I：

10.1007/s11696-021-01965-5

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Cercosporin has excellent properties of photosensitization that have been widely used in organophotocatalyst and photodynamic therapy as well as an antimicrobial agent. Therefore, there is a need to quantify it accurately with accessible methods. A comparative analysis of cercosporin quantification obtained by spectrophotometry (SPEC) and high-performance liquid chromatography (HPLC) was performed for nineteen Cercospora coffeicola isolates from different coffee-producing municipalities in Brazil. Image analysis of cercosporin crystals was performed in isolates with either high or low production of the toxin. Our results show that SPEC and HPLC are equally valid for the cercosporin evaluation of C. coffeicola cultures grown in vitro. The isolates with high cercosporin production had a higher crystal number and size when compared to the one with low cercosporin production.

引用

页码：2567 / 2572

页数：6

共 12 条

[1] Cercosporin production by Cercospora coffeicola isolates: spectrophotometry and HPLC quantification and image analysis
Deila Magna dos Santos Botelho
Mário Lúcio Vilela de Resende
Alexandre Rezende Teixeira
Wilder Douglas Santiago
Edson Ampélio Pozza
Silvino Intra Moreira
Juliana Barros Ramos
Sinara Oliveira de Aquino
Antônio Gilberto Ferreira
Tharyn Reichel
Leonor Guerra-Guimarães
Chemical Papers, 2022, 76 : 2567 - 2572
[2] Quantification of cercosporin from coffee leaves infected by Cercospora coffeicola
Juliana Barros Ramos
Mário Lúcio Vilela de Resende
Maria Eduarda Rodrigues Andrade
Alexandre Rezende Teixeira
Wilder Douglas Santiago
Edson Ampélio Pozza
Tharyn Reichel
Deila Magna dos Santos Botelho
Australasian Plant Pathology, 2022, 51 : 429 - 432
[3] Quantification of cercosporin from coffee leaves infected by Cercospora coffeicola
Ramos, Juliana Barros
Vilela de Resende, Mario Lucio
Rodrigues Andrade, Maria Eduarda
Teixeira, Alexandre Rezende
Santiago, Wilder Douglas
Pozza, Edson Ampelio
Reichel, Tharyn
dos Santos Botelho, Deila Magna
AUSTRALASIAN PLANT PATHOLOGY, 2022, 51 (04) : 429 - 432
[4] Temperature, incubation time and virulence of Cercospora coffeicola in the production of cercosporin
Souza Vale, Paula Adrielly
Vilela de Resende, Mario Lucio
dos Santos Botelho, Deila Magna
Pozza, Edson Ampelio
Ogoshi, Claudio
Andrade Monteiro, Ana Cristina
Garcia Costa, Bruno Henrique
Maia Vasconcelos, Victor Augusto
JOURNAL OF PHYTOPATHOLOGY, 2019, 167 (7-8) : 371 - 379
[5] CERCOSPORIN AND CBT PRODUCTION BY CERCOSPORA-BETICOLA ISOLATES
ASSANTE, G
DAMBRA, V
LOCCI, R
MUTTO, S
RIVISTA DI PATOLOGIA VEGETALE, 1981, 17 (1-2): : 91 - 97
[6] CERCOSPORIN PRODUCTION AND PATHOGENICITY OF CERCOSPORA-ARACHIDICOLA ISOLATES
MELOUK, HA
SCHUH, W
PHYTOPATHOLOGY, 1987, 77 (04) : 642 - 642
[7] Genetic Diversity of Cercospora kikuchii Isolates From Soybean Cultured in Argentina as Revealed by Molecular Markers and Cercosporin Production
Cristina Lura, Maria
Latorre Rapela, Maria Gabriela
Celia Vaccari, Maria
Maumary, Roxana
Soldano, Anabel
Mattio, Monica
Maria Gonzalez, Ana
MYCOPATHOLOGIA, 2011, 171 (05) : 361 - 371
[8] Genetic Diversity of Cercospora kikuchii Isolates From Soybean Cultured in Argentina as Revealed by Molecular Markers and Cercosporin Production
María Cristina Lurá
María Gabriela Latorre Rapela
María Celia Vaccari
Roxana Maumary
Anabel Soldano
Mónica Mattio
Ana María González
Mycopathologia, 2011, 171 : 361 - 371
[9] Biodiesel production from microalgal isolates of southern Pakistan and quantification of FAMEs by GC-MS/MS analysis
Syed Ghulam Musharraf
Muhammad Arif Ahmed
Noureen Zehra
Nurul Kabir
M Iqbal Choudhary
Atta-ur Rahman
Chemistry Central Journal, 6
[10] Biodiesel production from microalgal isolates of southern Pakistan and quantification of FAMEs by GC-MS/MS analysis
Musharraf, Syed Ghulam
Ahmed, Muhammad Arif
Zehra, Noureen
Kabir, Nurul
Choudhary, M. Iqbal
Rahman, Atta-ur
CHEMISTRY CENTRAL JOURNAL, 2012, 6

← 1 2 →