Scutellarin Blocks Sodium Current in Freshly Isolated Mouse Hippocampal CA1 Neurons

被引:5
|
作者
Zhang, Guangqin [1 ]
Qiu, Suhua [1 ]
Wei, Heming [2 ]
机构
[1] China Pharmaceut Univ, Dept Clin Pharm, Nanjing 210009, Peoples R China
[2] Natl Heart Ctr Singapore, Res & Dev Unit, Singapore 169612, Singapore
关键词
Scutellarin; Breviscapine; Hippocampal CA1 neurons; Na+ current; Patch clamp; DEPENDENT BLOCKER; CHANNEL BLOCKERS; BRAIN ISCHEMIA; NA+ CHANNELS; RAT; BREVISCAPINE; SYNAPTOSOMES; INHIBITION; PHENYTOIN; GLUTAMATE;
D O I
10.1007/s11064-011-0426-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Scutellarin (Scu), the main bioactive component of Erilgeron breviscapus, protects the brain against ischemic damages. To explore the therapeutic mechanism of Scu, we investigated the impact of Scu on sodium current (I (Na)) of freshly isolated mouse hippocampal CA1 neurons using the whole-cell patch clamp technique. Results showed that Scu inhibited I (Na) in concentration- and holding potential-dependent manners. At 50 mu M, Scu markedly shifted the steady state inactivation curve of I (Na) towards a more negative potential, slowed down the recovery of I (Na) from inactivation state, and elicited a frequency-dependent block of I (Na). The shape of the current-voltage (I-V) curve and the steady state activation curve of I (Na) were unaffected by Scu treatment. These findings suggest that Scu is capable of inhibiting I (Na) in neurons through predominantly affecting the inactivated state of I (Na). Inhibition of Na+ channels provides a novel pharmacological basis for the anti-ischemic application of Scu.
引用
收藏
页码:947 / 954
页数:8
相关论文
共 50 条
  • [41] INTERLEUKIN-2 MODULATES CALCIUM CURRENTS IN DISSOCIATED HIPPOCAMPAL CA1 NEURONS
    PLATASALAMAN, CR
    FFRENCHMULLEN, JMH
    NEUROREPORT, 1993, 4 (05) : 579 - 581
  • [42] Circadian Modulation of Neurons and Astrocytes Controls Synaptic Plasticity in Hippocampal Area CA1
    McCauley, John P.
    Petroccione, Maurice A.
    D'Brant, Lianna Y.
    Todd, Gabrielle C.
    Affinnih, Nurat
    Wisnoski, Justin J.
    Zahid, Shergil
    Shree, Swasti
    Sousa, Alioscka A.
    De Guzman, Rose M.
    Migliore, Rosanna
    Brazhe, Alexey
    Leapman, Richard D.
    Khmaladze, Alexander
    Semyanov, Alexey
    Zuloaga, Damian G.
    Migliore, Michele
    Scimemi, Annalisa
    CELL REPORTS, 2020, 33 (02):
  • [43] Bifemelane hydrochloride enhances 'ischemic tolerance' phenomenon in gerbil hippocampal CA1 neurons
    Ohtsuki, T
    Matsumoto, M
    Taguchi, A
    Yang, GM
    Mabuchi, T
    Matsushita, K
    Kuwabara, K
    Kitagawa, K
    Yanagihara, T
    LIFE SCIENCES, 1996, 59 (12) : 979 - 985
  • [44] Endocannabinoids Differentially Modulate Synaptic Plasticity in Rat Hippocampal CA1 Pyramidal Neurons
    Xu, Jian-Yi
    Chen, Rongqing
    Zhang, Jian
    Chen, Chu
    PLOS ONE, 2010, 5 (04):
  • [45] Inhibition of delayed rectifier K+ currents by copper in acutely dissociated rat hippocampal CA1 neurons
    Niu, Zhi-Dian
    Chen, Ju-Tao
    Wang, Shu
    Wang, Ming
    Li, Xin-Mei
    Ruan, Di-Yun
    TOXICOLOGY LETTERS, 2006, 165 (03) : 289 - 296
  • [46] Autocrine Boost of NMDAR Current in Hippocampal CA1 Pyramidal Neurons by a PMCA-Dependent, Perisynaptic, Extracellular pH Shift
    Chen, Huei-Ying
    Chesler, Mitchell
    JOURNAL OF NEUROSCIENCE, 2015, 35 (03) : 873 - 877
  • [47] Tetrahydroberberine inhibits acetylcholine-induced K+ current in acutely dissociated rat hippocampal CA1 pyramidal neurons
    Wu, J
    Jin, GZ
    NEUROSCIENCE LETTERS, 1997, 222 (02) : 115 - 118
  • [48] Serotonin-mediated modulation of Na+/K+ pump current in rat hippocampal CA1 pyramidal neurons
    Zhang, Li Nan
    Su, Su Wen
    Guo, Fang
    Guo, Hui Cai
    Shi, Xiao Lu
    Li, Wen Ya
    Liu, Xu
    Wang, Yong Li
    BMC NEUROSCIENCE, 2012, 13
  • [49] Transduction of hippocampal CA1 by adenovirus in vivo
    Kammesheidt, A
    Ito, KI
    Kato, K
    Villarreal, LP
    Sumikawa, K
    BRAIN RESEARCH, 1996, 736 (1-2) : 297 - 304
  • [50] Disruption of Dentate Gyrus Blocks Effect of Visual Input on Spatial Firing of CA1 Neurons
    Lee, Jong Won
    Kim, Woon Ryoung
    Sun, Woong
    Jung, Min Whan
    JOURNAL OF NEUROSCIENCE, 2012, 32 (38) : 12999 - 13003