31P NMR Spectroscopy Revealed Adenylate kinase-like Activity and Phosphotransferase-like Activity from F1-ATPase of Escherichia coli

被引：2
|
作者：
Kim, Hyun-Won [1 ,2 ]
机构：
[1] Yonsei Univ, Wonju Coll Med, Dept Biochem, Wonju 220701, South Korea
[2] Yonsei Univ, Wonju Coll Med, Inst Basic Med Sci, Wonju 220701, South Korea
来源：
BULLETIN OF THE KOREAN CHEMICAL SOCIETY | 2011年 / 32卷 / 01期
关键词：
P-31; NMR; F-1-ATPase; Escherichia coli; Adenylate kinase-like activity; Phosphotransferase-like activity; BOUND ADENOSINE-DIPHOSPHATE; NUCLEOTIDE-BINDING-SITES; MITOCHONDRIAL F1-ATPASE; ADENINE;
D O I：
10.5012/bkcs.2011.32.1.183
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Adenylate kinase-like activity and phosphotransferase-like activity from F-1-ATPase of Escherichia coli was revealed by P-31 NMR spectroscopy. Incubation of F-1-ATPase with ADP in the presence of Mg2+ shows the appearance of P-31 resonances from AMP and Pi, suggesting generation of AMP and ATP by adenylate kinase-like activity and the subsequent hydrolysis to Pi. Incubation of F-1-ATPase with ADP in the presence of methanol shows additional peak from methyl phosphate, suggesting phosphotransferase-like activity of F-1-ATPase. Both adenylate kinase-like activity and phosphotransferase-like activity has not been reported from F-1-ATPase of Escherichia coli. P-31 NMR could be a valuable tool for the investigation of phosphorous related enzyme.
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页码：183 / 185
页数：3
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