31P NMR Spectroscopy Revealed Adenylate kinase-like Activity and Phosphotransferase-like Activity from F1-ATPase of Escherichia coli

Adenylate kinase-like activity and phosphotransferase-like activity from F-1-ATPase of Escherichia coli was revealed by P-31 NMR spectroscopy. Incubation of F-1-ATPase with ADP in the presence of Mg2+ shows the appearance of P-31 resonances from AMP and Pi, suggesting generation of AMP and ATP by adenylate kinase-like activity and the subsequent hydrolysis to Pi. Incubation of F-1-ATPase with ADP in the presence of methanol shows additional peak from methyl phosphate, suggesting phosphotransferase-like activity of F-1-ATPase. Both adenylate kinase-like activity and phosphotransferase-like activity has not been reported from F-1-ATPase of Escherichia coli. P-31 NMR could be a valuable tool for the investigation of phosphorous related enzyme.