Expression and immunogenicity of the C-terminus of a major blood-stage surface protein of Plasmodium vivax, Pv200(19), secreted from Saccharomyces cerevisiae

被引：24

作者：

Kaslow, DC ^{[1
]}

Kumar, S ^{[1
]}

机构：

[1] NIAID,MALARIA VACCINES SECT,PARASIT DIS LAB,NIH,BETHESDA,MD 20892

来源：

IMMUNOLOGY LETTERS | 1996年 / 51卷 / 03期

关键词：

Plasmodium vivax; congenic; merozoite surface protein 1; vaccine;

D O I：

10.1016/0165-2478(96)02570-9

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The carboxy-terminus of the major merozoite surface protein of Plasmodium has been shown to be the target of protective immunity in a number of non-vivax malaria parasite species. In an effort to develop a protective vaccine for Plasmodium vivax, the most prevalent form of human malaria, we expressed in Saccharomyces cerevisiae the 19-kDa a carboxy-terminus of Pv200 as a His(6)-tagged, secreted polypeptide. Five of seven H-2 congenic mouse strains elicited antibodies that recognized yeast produced PV200(19) by ELISA. The vaccine appears to be immunogenic and widely recognized, and to contain one or more helper T cell epitopes that may allow boosting with subsequent natural infections.

引用

页码：187 / 189

页数：3

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