Identification of Individual Exosome-Like Vesicles by Surface Enhanced Raman Spectroscopy

被引：171

作者：

Stremersch, Stephan ^{[1
]}

Marro, Monica ^{[2
]}

Pinchasik, Bat-El ^{[3
]}

Baatsen, Pieter ^{[4
,5
]}

Hendrix, An ^{[6
]}

De Smedt, Stefaan C. ^{[1
]}

Loza-Alvarez, Pablo ^{[2
]}

Skirtach, Andre G. ^{[3
,7
,8
]}

Raemdonck, Koen ^{[1
]}

Braeckmans, Kevin ^{[8
,9
]}

机构：

[1] Univ Ghent, Fac Pharmaceut Sci, Lab Gen Biochem & Phys Pharm, Ottergemsesteenweg 460, B-9000 Ghent, Belgium

[2] Barcelona Inst Sci & Technol, ICFO Inst Ciencies Foton, Av Carl Friedrich Gauss 3, Barcelona 08860, Spain

[3] Max Planck Inst Colloids & Interfaces, Dept Biomat, Dept Interfaces, Muhlenberg 1 OT Golm, D-14476 Potsdam, Germany

[4] KULeuven, VIB KULeuven Bio Imaging Core, EM Facil EMoNe, Herestr 49, B-3000 Leuven, Belgium

[5] KULeuven, Ctr Human Genet, Herestr 49, B-3000 Leuven, Belgium

[6] Ghent Univ Hosp, Dept Radiat Oncol & Expt Canc Res, Lab Expt Canc Res, De Pintelaan 185, B-900 Ghent, Belgium

[7] Univ Ghent, Dept Mol Biotechnol, Coupure Links 653, B-9000 Ghent, Belgium

[8] Univ Ghent, Ctr Nano & Biophoton, B-9000 Ghent, Belgium

[9] Univ Ghent, Fac Pharmaceut Sci, Dept Pharmaceut, Ottergemsesteenweg 460, B-9000 Ghent, Belgium

来源：

SMALL | 2016年 / 12卷 / 24期

基金：

比利时弗兰德研究基金会;

关键词：

RED-BLOOD-CELLS; EXTRACELLULAR VESICLES; BIOANALYTICAL APPLICATIONS; GOLD NANOPARTICLES; SINGLE-MOLECULE; SERS; SCATTERING; PLASMA; RNA; MICROVESICLES;

D O I：

10.1002/smll.201600393

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Exosome-like vesicles (ELVs) are a novel class of biomarkers that are receiving a lot of attention for the detection of cancer at an early stage. In this study the feasibility of using a surface enhanced Raman spectroscopy (SERS) based method to distinguish between ELVs derived from different cellular origins is evaluated. A gold nanoparticle based shell is deposited on the surface of ELVs derived from cancerous and healthy cells, which enhances the Raman signal while maintaining a colloidal suspension of individual vesicles. This nanocoating allows the recording of SERS spectra from single vesicles. By using partial least squares discriminant analysis on the obtained spectra, vesicles from different origin can be distinguished, even when present in the same mixture. This proof-of-concept study paves the way for noninvasive (cancer) diagnostic tools based on exosomal SERS fingerprinting in combination with multivariate statistical analysis.

引用

页码：3292 / 3301

页数：10

共 54 条

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