Functional characterization of a panel of high-grade serous ovarian cancer cell lines as representative experimental models of the disease

被引:59
作者
Haley, James [1 ]
Tomar, Sunil [1 ]
Pulliam, Nicholas [1 ]
Xiong, Sen [1 ]
Perkins, Susan M. [2 ]
Karpf, Adam R. [3 ,4 ]
Mitra, Sumegha [1 ,5 ]
Nephew, Kenneth P. [1 ,6 ,7 ]
Mitra, Anirban K. [1 ,6 ,8 ]
机构
[1] Indiana Univ Sch Med, Med Sci Program, Bloomington, IN 47405 USA
[2] Indiana Univ, Dept Biostat, Indianapolis, IN 46202 USA
[3] Univ Nebraska Med Ctr, Eppley Inst, Omaha, NE 68198 USA
[4] Univ Nebraska Med Ctr, Fred & Pamela Buffett Canc Ctr, Omaha, NE 68198 USA
[5] Indiana Univ Sch Med, Dept Biochem & Mol Biol, Indianapolis, IN 46202 USA
[6] Indiana Univ, Melvin & Bren Simon Canc Ctr, Indianapolis, IN 46202 USA
[7] Indiana Univ Sch Med, Dept Cellular & Integrat Physiol, Indianapolis, IN 46202 USA
[8] Indiana Univ Sch Med, Dept Med & Mol Genet, Indianapolis, IN 46202 USA
基金
美国国家卫生研究院;
关键词
ovarian cancer; migration; invasion; proliferation; clonogenicity; METASTASIS; INHIBITOR; INSIGHTS;
D O I
10.18632/oncotarget.9053
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Genomic analysis of ovarian cancer cell lines has revealed a panel that best represents the most common ovarian cancer subtype, high-grade serous ovarian cancer (HGSOC). However, these HGSOC-like cell lines have not been extensively applied by ovarian cancer researchers to date, and the most commonly used cell lines in the ovarian cancer field do not genetically resemble the major clinical type of the disease. For the HGSOC-like lines to serve as suitable models, they need to be characterized for common functional assays. To achieve that objective, we systematically studied a panel of HGSOC cells CAOV3, COV362, Kuramochi, OVCAR4, OVCAR5, OVCAR8, OVSAHO and SNU119 for migration, invasion, proliferation, clonogenicity, EMT phenotype and cisplatin resistance. They exhibited a range of efficacies and OVCAR5, OVCAR8 and Kuramochi were the most aggressive. SNU119 and OVSAHO cells demonstrated the lowest functional activities. Wide differences in expression of EMT markers were observed between cell lines. SNU119 were the most epithelial and OVCAR8 had the most mesenchymal phenotype. COV362 was the most resistant to cisplatin while CAOV3 was the most sensitive. Taken together, our systematic characterization represents a valuable resource to help guide the application of HGSOC cells by the cancer research community.
引用
收藏
页码:32810 / 32820
页数:11
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