Bicontinuous Microemulsion as Reaction Medium for ω-Transaminase Catalysed Biotransformations

被引：8

作者：

Laupheimer, M. ^{[1
]}

Engelskirchen, S. ^{[1
]}

Tauber, K. ^{[2
]}

Kroutil, W. ^{[2
]}

Stubenrauch, C. ^{[1
]}

机构：

[1] Univ Stuttgart, Inst Phys Chem, D-70569 Stuttgart, Germany

[2] Karl Franzens Univ Graz, Inst Chem Organ & Bioorgan Chem, Graz, Austria

来源：

TENSIDE SURFACTANTS DETERGENTS | 2011年 / 48卷 / 01期

关键词：

Bicontinuous microemulsion; biotransformation; enzyme reaction; enzyme-substrate incompatibility; reaction medium; IN-OIL MICROEMULSIONS; WATER; LIPASE; KINETICS; MICELLAR;

D O I：

10.3139/113.110100

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Due to their high enantioselectivity biotransformations, i.e. enzyme-catalysed conversion of organic compounds, are extremely attractive reactions. However, a limiting factor for choosing substrates is the enzyme-substrate incompatibility. This occurs when a hydrophilic enzyme which naturally resides in the aqueous cell cytoplasm is supposed to convert a hydrophobic substrate. In this context bicontinuous microemulsions appear to be a beneficial reaction medium for biotransformations, particularly due to their large interfacial area between a hydrophilic and a hydrophobic compound. As a "proof of concept" we performed omega-transaminase (EC 2.6.1.18) catalysed model reactions in a bicontinuous microemulsion of the type phosphate buffer/NaCl - n-octane - pentaethylene glycol monodecyl ether.

引用

页码：28 / 33

页数：6