Amplification strategy based on gold nanoparticle-decorated carbon nanotubes for neomycin immunosensors

被引:60
|
作者
Zhu, Ye [1 ]
Son, Jung Ik [1 ]
Shim, Yoon-Bo [1 ]
机构
[1] Pusan Natl Univ, Dept Chem, Pusan 609735, South Korea
来源
BIOSENSORS & BIOELECTRONICS | 2010年 / 26卷 / 03期
关键词
Immunosensor; Neomycin; Conducting polymer; Gold nanoparticle; Carbon nanotube; Hydrazine; CONDUCTING POLYMER; ELECTROCHEMICAL DETECTION; DNA; ELECTRODES; BIOSENSOR; PROTEINS; TRANSDUCTION; HYDRAZINE; ASSAY;
D O I
10.1016/j.bios.2010.08.023
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
A novel amperometric immunosensor with an enhanced sensitivity for the detection of neomycin (Neo) was prepared by covalently immobilizing a monoclonal Neo antibody onto a new conducting polymer, poly-[2,5-di-(2-thienyl)-1H-pyrrole-1-(p-benzoic acid)] (pDPB), as a sensor probe. The probe was used to detect Neo in a sandwich-type approach, where the secondary antibody was attached to gold nanoparticle-decorated multi-wall carbon nanotubes labeled with hydrazine (Hyd-MWCNT(AuNP)-Ab(2)). Hydrazine on the conjugate served as a catalyst for the reduction of hydrogen peroxide, and the catalytic current was monitored at -0.45 V vs. Ag/AgCl. The performance of the immunosensor with and without AuNPs on the probe and the conjugate was compared. The parameters affecting the immunosensor response in terms of antibody dilution ratio, incubation time, pH, applied potential, and temperature were optimized. A linear dynamic range for Neo analysis was obtained between 10 ng/mL and 250 ng/mL with a detection limit of 6.76 +/- 0.17 ng/ml.. The proposed immunosensor was successfully applied to detect Neo content in real meat samples. (C) 2010 Elsevier BM. All rights reserved.
引用
收藏
页码:1002 / 1008
页数:7
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