Screening of protein crystallization conditions on a microfluidic chip using nanoliter-size droplets

被引：601

作者：

Zheng, B ^{[1
]}

Roach, LS ^{[1
]}

Ismagilov, RF ^{[1
]}

机构：

[1] Univ Chicago, Dept Chem, Chicago, IL 60637 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2003年 / 125卷 / 37期

关键词：

D O I：

10.1021/ja037166v

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Protein crystallization is a major bottleneck in determining tertiary protein structures from genomic sequence data. This paper describes a microfluidic system for screening hundreds of protein crystallization conditions using less than 4 nL of protein solution for each crystallization droplet. The droplets are formed by mixing protein, precipitant, and additive stock solutions in variable ratios in a flow of water-immiscible fluids inside microchannels. Each droplet represents a discrete trial testing different conditions. The system has been validated by crystallization of several water-soluble proteins. Copyright © 2003 American Chemical Society.

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页码：11170 / 11171

页数：2

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