A "Clickable" Electrodeposited Polymer Films Based on 3-ethynylthiophene for the Covalent Immobilization of Proteins. Application to a Label-free Electrochemical Immunosensor for Escherichia Coli and Staphylococcus Aureus Determination

被引：6

作者：

Svalova, T. S. ^{[1
]}

Medvedeva, M., V ^{[1
]}

Kozitsina, A. N. ^{[1
]}

机构：

[1] Ural Fed Univ, Inst Chem Technol, Ekaterinburg 620002, Russia

来源：

ELECTROANALYSIS | 2021年 / 33卷 / 12期

关键词：

Electrochemical polymerization; 3-ethynylthiophene; 3; 4-ethylendioxythiophene; layer-by-layer electrodeposition; copper catalysed azide-alkyne cycloaddition; covalent immobilization of proteins; label-free electrochemical immunosensor;

D O I：

10.1002/elan.202100320

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An original electrodeposited polymer film, based on 3-ethynylthiophene, for covalent immobilization of proteins via "click" reaction of copper catalyzed azide-alkyne cycloaddition was developed. The best characteristics have demonstrated the layer-by-layer immobilized 3,4-ethylendioxythiophene-3-ethynylthiophene composite film. The bovine serum albumin and antibodies have been successfully immobilized on the surface of planar platinum electrode. The approach has been applied to label-free electrochemical immunosensors for Escherichia coli and Staphylococcus aureus determination. The immunosensors demonstrated LODs 7.2 CFU/mL for Escherichia coli and 15.9 CFU/mL for Staphylococcus aureus, linear range 10(2)-10(6) CFU/mL and could be promising for environmental and food control.

引用

页码：2469 / 2475

页数：7

共 24 条

[1] Rapid and sensitive detection of Salmonella with reduced graphene oxide-carbon nanotube based electrochemical aptasensor [J].

Appaturi, Jimmy Nelson ;

Pulingam, Thiruchelvi ;

Thong, Kwai Lin ;

Muniandy, Shalini ;

Ahmad, Noraini ;

Leo, Bey Fen .

ANALYTICAL BIOCHEMISTRY, 2020, 589

[2] Electrochemical impedance immunosensor for rapid detection of stressed pathogenic Staphylococcus aureus bacteria [J].

Bekir, Karima ;

Barhoumi, Houcine ;

Braiek, Mohamed ;

Chrouda, Amani ;

Zine, Nadia ;

Abid, Nabil ;

Maaref, Abdelrazek ;

Bakhrouf, Amina ;

Ben Ouada, Hafedh ;

Jaffrezic-Renault, Nicole ;

Ben Mansour, Hedi .

ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH, 2015, 22 (20) :15796-15803

[3] Biosensing strategies for the electrochemical detection of viruses and viral diseases e A review [J].

Brazaca, Lais Canniatti ;

dos Santos, Pamyla Layene ;

de Oliveira, Paulo Roberto ;

Rocha, Diego Pessoa ;

Stefano, Jessica Santos ;

Kalinke, Cristiane ;

Munoz, Rodrigo Alejandro Abarza ;

Bonacin, Juliano Alves ;

Janegitz, Bruno Campos ;

Carrilho, Emanuel .

ANALYTICA CHIMICA ACTA, 2021, 1159

[4] An electrochemical biosensor based on methylene blue-loaded nanocomposites as signal-amplifying tags to detect pathogenic bacteria [J].

Bu, Shengjun ;

Wang, Kuiyu ;

Li, Zhongyi ;

Wang, Chengyun ;

Hao, Zhuo ;

Liu, Wensen ;

Wan, Jiayu .

ANALYST, 2020, 145 (12) :4328-4334

[5] Electrochemical Affinity Biosensors Based on Selected Nanostructures for Food and Environmental Monitoring [J].

Campuzano, Susana ;

Yanez-Sedeno, Paloma ;

Pingarron, Jose M. .

SENSORS, 2020, 20 (18) :1-28

[6]

Evtugyn G, 2015, ADV MATER SER, P89

[7] Well-Orientation Strategy for Direct Immobilization of Antibodies: Development of the Immunosensor Using the Boronic Acid-Modified Magnetic Graphene Nanoribbons for Ultrasensitive Detection of Lymphoma Cancer Cells [J].

Hashemi, Pegah ;

Afkhami, Abbas ;

Baradaran, Behzad ;

Halabian, Raheleh ;

Madrakian, Tayyebeh ;

Arduini, Fabiana ;

Tien Anh Nguyen ;

Bagheri, Hasan .

ANALYTICAL CHEMISTRY, 2020, 92 (16) :11405-11412

[8] Copper-catalyzed azide-alkyne cycloaddition (CuAAC) and beyond: new reactivity of copper(I) acetylides [J].

Hein, Jason E. ;

Fokin, Valery V. .

CHEMICAL SOCIETY REVIEWS, 2010, 39 (04) :1302-1315

[9] Reduced graphene oxide/polyethylenimine based immunosensor for the selective and sensitive electrochemical detection of uropathogenic Escherichia coli [J].

Jijie, Roxana ;

Kahlouche, Karima ;

Barras, Alexandre ;

Yamakawa, Nao ;

Bouckaert, Julie ;

Gharbi, Tijani ;

Szunerits, Sabine ;

Boukherroub, Rabah .

SENSORS AND ACTUATORS B-CHEMICAL, 2018, 260 :255-263

[10] Sensors Based on Bio and Biomimetic Receptors in Medical Diagnostic, Environment, and Food Analysis [J].

Kozitsina, Alisa N. ;

Svalova, Tatiana S. ;

Malysheva, Natalia N. ;

Okhokhonin, Andrei V. ;

Vidrevich, Marina B. ;

Brainina, Khiena Z. .

BIOSENSORS-BASEL, 2018, 8 (02)

← 1 2 3 →