Genes transactivated by hepatitis C virus core protein, a microarray assay

被引：7

作者：

Liu, Min ^{[1
]}

Zhang, Shu-Lin ^{[1
]}

Cheng, Jun ^{[2
]}

Liu, Yan ^{[2
]}

Wang, Lin ^{[2
]}

Shao, Qing ^{[2
]}

Zhang, Jian ^{[2
]}

Lin, Shu-Mei ^{[1
]}

机构：

[1] Xi An Jiao Tong Univ, Affiliated Hosp 1, Dept Infect Dis, Coll Med, Xian 710061, Shaanxi Provinc, Peoples R China

[2] 302 Hosp PLA, Gene Therapy Res Ctr, Inst Infect Dis, Beijing 100039, Peoples R China

来源：

WORLD JOURNAL OF GASTROENTEROLOGY | 2005年 / 11卷 / 22期

基金：

中国国家自然科学基金;

关键词：

Hepatitis C virus; Core protein; Microarray assay;

D O I：

10.3748/wjg.v11.i22.3351

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

AIM: To explore the new target genes transactivated by hepatitis C virus (HCV) core protein and to elucidate the pathogenesis of HCV infection. METHODS: Reverse transcribed cDNA was subjected to microarray assay. The coding gene transactivated by HCV core protein was cloned and analyzed with bioinformatics methods. RESULTS: The expressive vector of pcDNA3.1(-)-core was constructed and confirmed by restriction enzyme digestion and DNA sequencing and approved correct. mRNA was purified from HepG2 and HepG2 cells transfected with pcDNA3.1(-)-core, respectively. The cDNA derived was subjected to microarray assay. A new gene named HCTP4 was cloned with molecular biological method in combination with bioinformatics method. CONCLUSION: HCV core is a potential transactivator. Microarray is an efficient and convenient method for analysis of differentially expressed genes. (C) 2005 The WJG Press and Elsevier Inc. All rights reserved.

引用

页码：3351 / 3356

页数：6

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