Occurrence of Atypical and New Genotypes of Toxoplasma Gondii in Free-Range Chickens Intended for Human Consumption in Brazil

被引：7
作者：
dos Santos Silva, Ana Clecia [1 ,2 ]
de Barros, Luiz Daniel [1 ]
Barros, Viviane Melo Coelho [2 ]
de Alcantara, Adrianne Mota [3 ]
Andrade, Muller Ribeiro [3 ]
Garcia, Joao Luis [1 ]
Mota, Rinaldo Aparecido [3 ]
Porto, Wagnner Jose Nascimento [2 ]
机构：
[1] Univ Estadual Londrina, Lab Anim Protozool, Dept Vet Prevent Med, Rodovia Celso Garcia Cid,Pr 445 Km 380, BR-86057970 Londrina, PR, Brazil
[2] Univ Fed Alagoas, Lab Vet Parasitol, Fazenda Sao Luis S-N, BR-57700970 Vicosa, AL, Brazil
[3] Univ Fed Rural Pernambuco, Lab Infect Dis Domest Anim, Rua Dom Manoel de Medeiros,S-N Dois Irmaos, BR-52171900 Recife, PE, Brazil
来源：
ACTA PARASITOLOGICA | 2020年 / 65卷 / 03期
关键词：
Toxoplasmosis; Genotyping; PCR-RFLP; Zoonosis; FERNANDO-DE-NORONHA; GENETIC-CHARACTERIZATION; GALLUS-DOMESTICUS; INFECTION; NORTHEAST; VIRULENCE; STATE; CATS; PCR; IDENTIFICATION;
D O I：
10.2478/s11686-020-00194-2
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Purpose The aim of the present study was to detect and genotype T. gondii in free-range chickens destined to human consumption in Alagoas state, Brazil. Methods Two hundred blood samples were collected from free-range chickens and submitted to indirect immunofluorescence antibody test (IFAT). Brain tissue from 14 animals randomly selected were subjected to mouse bioassay. Positive samples in mouse bioassay were submitted to PCR and genotyped by PCR-RFLP. Results Out of two hundred blood samples from chickens, 72 (36%) samples were considered positive by IFAT. Two T. gondii strains were isolated, both being characterized as atypical and classified as #146 and a new genotype, named #279 in ToxoDB database. Conclusions Our results showed a sero-occurrence of T. gondii in free-range chickens intended for humans, and the genetic diversity of the parasite in Brazil, with a new genotype described.
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收藏
页码：774 / 778
页数：5
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