PCR-REA as an important tool for the identification of Cryptococcus neoformans and Cryptococcus gattii from human and veterinary sources

被引：6

作者：

Cordeiro, Rossana Aguiar ^{[1
,2
]}

Freire Costa, Ana Karoline ^{[3
]}

Nogueira Brilhante, Raimunda Samia ^{[1
,2
]}

Chaves de Lima, Rita Amanda ^{[2
]}

Collares Maia Castelo-Branco, Debora de Souza ^{[2
]}

Ribeiro, Joyce Fonteles ^{[2
]}

Monteiro, Andre Janes ^{[4
]}

Castro Rocha, Francisco Airton ^{[5
]}

Costa Sidrim, Jose Julio ^{[1
,2
]}

Gadelha Rocha, Marcos Fabio ^{[1
,2
,3
]}

机构：

[1] Univ Fed Ceara, Dept Pathol & Legal Med, Sch Med, Specialized Med Mycol Ctr, BR-60430275 Fortaleza, Ceara, Brazil

[2] Univ Fed Ceara, Dept Pathol & Legal Med, Sch Med, Postgrad Program Med Microbiol, BR-60430275 Fortaleza, Ceara, Brazil

[3] Univ Estadual Ceara, Sch Vet Med, Postgrad Program Vet Sci, Fortaleza, Ceara, Brazil

[4] Univ Fed Ceara, Dept Stat, BR-60430275 Fortaleza, Ceara, Brazil

[5] Univ Fed Ceara, Dept Internal Med, Fac Med, BR-60430275 Fortaleza, Ceara, Brazil

来源：

VETERINARY MICROBIOLOGY | 2011年 / 154卷 / 1-2期

关键词：

Cryptococcus; Identification; PCR-REA; API; 20C; VITEK; INFECTION; GENOTYPE; YEASTS;

D O I：

10.1016/j.vetmic.2011.06.038

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The extraordinary ability of Cryptococcus species to cause disease has focused the attention of scientists on finding ways to improve their identification methods. In this study. PCR-REA, manual methods (morphological and biochemical characteristics), API 20C and VITEK 2 were used to test identify a total of 30 Cryptococcus spp. from human and veterinary sources. PCR-REA was performed using the capsular region as amplification target followed by restriction with the enzymes AgeI, BsmFI and HpaII. PCR-REA identified the strains as C. neoformans var. grubii (n = 19) and C. gattii (n = 8). There was no significant difference between the API 20C AUX and VITEK 2 when compared to manual methods for the identification of Cryptococcus spp. However, none of these non-manual methods were able to detect C. gattii samples. PCR-REA showed a greater level of concordance with the manual method, besides being faster and more sensitive than the other methods. Therefore, it is indicated for routine identification of Cryptococcus spp. strains. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：180 / 184

页数：5

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