Colorimetric detection of α-glucosidase activity based on the etching of gold nanorods and its application to screen anti-diabetic drugs

被引：48
作者：
Cheng, Xin [1 ]
Huang, Yan [1 ]
Yuan, Chao [1 ]
Dai, Ke [2 ]
Jiang, Hong [1 ]
Ma, Jimei [1 ]
机构：
[1] Huazhong Agr Univ, Coll Sci, Dept Chem, Wuhan 430070, Hubei, Peoples R China
[2] Huazhong Agr Univ, Coll Resources & Environm, Wuhan 430070, Hubei, Peoples R China
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2019年 / 282卷 / 838-843期
关键词：
Colorimetric method; Gold nanorods; Etching; alpha-Glucosidase; Inhibitor screening; Visual detection; INHIBITOR; SENSOR; ASSAYS;
D O I：
10.1016/j.snb.2018.11.097
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
An ultrasensitive colorimetric method for alpha-glucosidase sensing based on iodine-mediated etching of gold nanorods (AuNRs) was developed. In this method, 2-O-alpha-D-glucopyranosyl-L-ascorbic acid (AA-2G) was used as an enzymatic substrate. The hydrolysate ascorbic acid in situ reduced KIO3 to I-2, which subsequently etched the AuNRs. A blue-shift of the localized surface plasmon resonance (LSPR) absorption of AuNRs was clearly observed by UV-vis spectroscopy and reflected with visual color changes. The shift showed a good linear relationship with the concentration of alpha-glucosidase during 2.5-45 U/L and a low alpha-glucosidase detection limit of 0.5 U/L was provided. This strategy was featured with low cost, simplicity, excellent sensitivity, high selectivity and successfully applied in the alpha-glucosidase inhibitor screening with Chinese Wolfberries as candidates.
引用
收藏
页码：838 / 843
页数：6
相关论文
共 39 条
[21]   Penasulfate A, a new α-glucosidase inhibitor from a marine sponge Penares sp. [J].
Nakao, Y ;
Maki, T ;
Matsunaga, S ;
van Soest, RWM ;
Fusetani, N .
JOURNAL OF NATURAL PRODUCTS, 2004, 67 (08) :1346-1350
[22]   Ultrasensitive colorimetric detection of Cu2+ using gold nanorods [J].
Niu, Xiaofang ;
Xu, Dong ;
Yang, Yunhui ;
He, Yan .
ANALYST, 2014, 139 (11) :2691-2694
[23]   A simple and efficient synthesis of novel inhibitors of alpha-glucosidase based on benzimidazole skeleton and molecular docking studies [J].
Ozil, Musa ;
Emirik, Mustafa ;
Etlik, Semiha Yilmaz ;
Ulker, Serdar ;
Kahveci, Bahittin .
BIOORGANIC CHEMISTRY, 2016, 68 :226-235
[24]   Hypoglycemic action of borapetoside A from the plant Tinospora crispa in mice [J].
Ruan, Chi-Tun ;
Lam, Sio-Hong ;
Lee, Shoei-Sheng ;
Su, Ming-Jai .
PHYTOMEDICINE, 2013, 20 (8-9) :667-675
[25]   Enzymatic etching of gold nanorods by horseradish peroxidase and application to blood glucose detection [J].
Saa, Laura ;
Coronado-Puchau, Marc ;
Pavlov, Valeri ;
Liz-Marzan, Luis M. .
NANOSCALE, 2014, 6 (13) :7405-7409
[26]   Seeded high yield synthesis of short Au nanorods in aqueous solution [J].
Sau, TK ;
Murphy, CJ .
LANGMUIR, 2004, 20 (15) :6414-6420
[27]   Hydroxycoumarin Derivatives: Novel and Potent α-Glucosidase Inhibitors [J].
Shen, Qiong ;
Shao, Jialiang ;
Peng, Quan ;
Zhang, Wanjin ;
Ma, Lin ;
Chan, Albert S. C. ;
Gu, Lianquan .
JOURNAL OF MEDICINAL CHEMISTRY, 2010, 53 (23) :8252-8259
[28]   A fluorometric and real-time assay for a-glucosidase activity through supramolecular self-assembly and its application for inhibitor screening [J].
Tang, Cong ;
Qian, Zhaosheng ;
Qian, Yinjie ;
Huang, Yuanyuan ;
Zhao, Meizhi ;
Ao, Hang ;
Feng, Hui .
SENSORS AND ACTUATORS B-CHEMICAL, 2017, 245 :282-289
[29]   Label-free colorimetric sensing of copper(II) ions based on accelerating decomposition of H2O2 using gold nanorods as an indicator [J].
Wang, Shasha ;
Chen, Zhaopeng ;
Chen, Ling ;
Liu, Ruili ;
Chen, Lingxin .
ANALYST, 2013, 138 (07) :2080-2084
[30]   A dual-mode probe for colorimetric and fluorometric detection of cysteine based on phosphorus/nitrogen co-doped CQDs and gold nanorods [J].
Wang, Yanying ;
Liang, Ruoxuan ;
Liu, Wei ;
Zhao, Qingbiao ;
Zhu, Xiaoyan ;
Yang, Li ;
Zou, Ping ;
Wang, Xianxiang ;
Ding, Fang ;
Rao, Hanbing .
SENSORS AND ACTUATORS B-CHEMICAL, 2018, 273 :1627-1634
1234