Colorimetric detection of α-glucosidase activity based on the etching of gold nanorods and its application to screen anti-diabetic drugs

被引：48
作者：
Cheng, Xin [1 ]
Huang, Yan [1 ]
Yuan, Chao [1 ]
Dai, Ke [2 ]
Jiang, Hong [1 ]
Ma, Jimei [1 ]
机构：
[1] Huazhong Agr Univ, Coll Sci, Dept Chem, Wuhan 430070, Hubei, Peoples R China
[2] Huazhong Agr Univ, Coll Resources & Environm, Wuhan 430070, Hubei, Peoples R China
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2019年 / 282卷 / 838-843期
关键词：
Colorimetric method; Gold nanorods; Etching; alpha-Glucosidase; Inhibitor screening; Visual detection; INHIBITOR; SENSOR; ASSAYS;
D O I：
10.1016/j.snb.2018.11.097
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
An ultrasensitive colorimetric method for alpha-glucosidase sensing based on iodine-mediated etching of gold nanorods (AuNRs) was developed. In this method, 2-O-alpha-D-glucopyranosyl-L-ascorbic acid (AA-2G) was used as an enzymatic substrate. The hydrolysate ascorbic acid in situ reduced KIO3 to I-2, which subsequently etched the AuNRs. A blue-shift of the localized surface plasmon resonance (LSPR) absorption of AuNRs was clearly observed by UV-vis spectroscopy and reflected with visual color changes. The shift showed a good linear relationship with the concentration of alpha-glucosidase during 2.5-45 U/L and a low alpha-glucosidase detection limit of 0.5 U/L was provided. This strategy was featured with low cost, simplicity, excellent sensitivity, high selectivity and successfully applied in the alpha-glucosidase inhibitor screening with Chinese Wolfberries as candidates.
引用
收藏
页码：838 / 843
页数：6
相关论文
共 39 条
[1]   Gold nanorod-based electrochemical sensing of small biomolecules: A review [J].
Alagiri, Mani ;
Rameshkumar, Perumal ;
Pandikumar, Alagarsamy .
MICROCHIMICA ACTA, 2017, 184 (09) :3069-3092
[2]  
[Anonymous], SCI REP
[3]   Novel Fluorescent Biosensor for α-Glucosidase Inhibitor Screening Based on Cationic Conjugated Polymers [J].
Cao, Ali ;
Tang, Yanli ;
Liu, Yue .
ACS APPLIED MATERIALS & INTERFACES, 2012, 4 (08) :3773-3778
[4]   Sensitive colorimetric assays for α-glucosidase activity and inhibitor screening based on unmodified gold nanoparticles [J].
Chen, Hongxia ;
Zhang, Jiangjiang ;
Wu, Heng ;
Koh, Kwangnak ;
Yin, Yongmei .
ANALYTICA CHIMICA ACTA, 2015, 875 :92-98
[5]   Colorimetric sensing of copper(II) based on catalytic etching of gold nanorods [J].
Chen, Zhaopeng ;
Liu, Ruili ;
Wang, Shasha ;
Qu, Chengli ;
Chen, Lingxin ;
Wang, Zhuo .
RSC ADVANCES, 2013, 3 (32) :13318-13323
[6]   Highly sensitive label-free colorimetric sensing of nitrite based on etching of gold nanorods [J].
Chen, Zhaopeng ;
Zhang, Zhiyang ;
Qu, Chengli ;
Pan, Dawei ;
Chen, Lingxin .
ANALYST, 2012, 137 (22) :5197-5200
[7]   α-Glucosidase Inhibitors from Brickellia cavanillesii [J].
Escandon-Rivera, Sonia ;
Gonzalez-Andrade, Martin ;
Bye, Robert ;
Linares, Edelmira ;
Navarrete, Andres ;
Mata, Rachel .
JOURNAL OF NATURAL PRODUCTS, 2012, 75 (05) :968-974
[8]   Carbon dots for fluorescent detection of α-glucosidase activity using enzyme activated inner filter effect and its application to anti-diabetic drug discovery [J].
Kong, Weiheng ;
Wu, Di ;
Xia, Lian ;
Chen, Xuefeng ;
Li, Guoliang ;
Qiu, Nannan ;
Chen, Guang ;
Sun, Zhiwei ;
You, Jinmao ;
Wu, Yongning .
ANALYTICA CHIMICA ACTA, 2017, 973 :91-99
[9]   Non-aggregation based label free colorimetric sensor for the detection of Cr (VI) based on selective etching of gold nanorods [J].
Li, Fei-Ming ;
Liu, Jia-Ming ;
Wang, Xin-Xing ;
Lin, Li-Ping ;
Cai, Wen-Lian ;
Lin, Xuan ;
Zeng, Yi-Na ;
Li, Zhi-Ming ;
Lin, Shao-Qin .
SENSORS AND ACTUATORS B-CHEMICAL, 2011, 155 (02) :817-822
[10]   A fluorescence resonance energy transfer (FRET) based "Turn-On" nanofluorescence sensor using a nitrogen-doped carbon dot-hexagonal cobalt oxyhydroxide nanosheet architecture and application to α-glucosidase inhibitor screening [J].
Li, Guoliang ;
Kong, Weiheng ;
Zhao, Mei ;
Lu, Shuaimin ;
Gong, Peiwei ;
Chen, Guang ;
Xia, Lian ;
Wang, Hua ;
You, Jinmao ;
Wu, Yongning .
BIOSENSORS & BIOELECTRONICS, 2016, 79 :728-735
1234