Comparison of some centrifugal partition chromatography systems for a general separation of plant extracts

被引：12
|
作者：
Ingkaninan, K [1 ]
Hermans-Lokkerbol, ACJ [1 ]
Verpoorte, R [1 ]
机构：
[1] Leiden Univ, Gorlaeus Labs, Leiden Amsterdam Ctr Drug Res, Div Pharmacognosy, NL-2300 RA Leiden, Netherlands
来源：
JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 1999年 / 22卷 / 06期
关键词：
D O I：
10.1081/JLC-100101705
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
In order to find a Centrifugal Partition Chromatography (CPC) solvent system that can be used as a general prefractionation step for crude plant extracts, twelve two-phase systems were compared. The butanol: ethyl acetate: water 3:2:5 (v/v/v) system gave good separation for polar compounds, and the heptane: ethyl acetate: methanol: water 6:1:6:1 (v/v/v/v) system was found to be effective for the separation of nonpolar compounds and somewhat less for polar compounds.
引用
收藏
页码：885 / 896
页数：12
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