Effects of light intensity and nitrogen starvation on growth, total fatty acids and arachidonic acid in the green microalga Parietochloris incisa

被引：244
作者：
Solovchenko, A. E. [1 ]
Khozin-Goldberg, I. [2 ]
Didi-Cohen, S. [2 ]
Cohen, Z. [2 ]
Merzlyak, M. N. [1 ]
机构：
[1] Moscow MV Lomonosov State Univ, Dept Physiol Microorganisms, Fac Biol, Moscow 119991, Russia
[2] Ben Gurion Univ Negev, Jacob Blaustein Inst Desert Res, Microalgal Biotechnol Lab, Albert Katz Dept Dryland Biotechnol, IL-84990 Sede Boqer, Israel
来源：
JOURNAL OF APPLIED PHYCOLOGY | 2008年 / 20卷 / 03期
基金：
俄罗斯基础研究基金会;
关键词：
alga cultivation; lipids; microalgal biotechnology; photoadaptation;
D O I：
10.1007/s10811-007-9233-0
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The effects of light and nitrogen deficiency on biomass, fatty acid content and composition were studied in Parietochloris incisa, the unicellular freshwater chlorophyte accumulating very high amounts of arachidonic-acid-rich triacylglycerols. P. incisa cultures grown on complete nutrient medium and under high light (400 mu mol photons m(-2) s(-1)) showed the highest rate of growth in comparison to medium (200 mu mol photons m(-2) s(-1)) and low (35 mu mol photons m(-2) s(-1)) light intensity. Cultures grown under high light (on complete BG-11 medium) attained higher volumetric contents of total fatty acids and arachidonic acid due to greater increase in biomass. Nitrogen starvation brought about a strong increase in the arachidonic acid proportion of total fatty acids. Thus, adjustments to cultivation conditions could serve as an efficient tool for manipulation of yield and relative content of arachidonic acid in P. incisa. The significance of the changes in lipid metabolism for adaptation of P. incisa to high-light stress and nitrogen deficiency is also discussed.
引用
收藏
页码：245 / 251
页数：7
相关论文
共 30 条
[11]   Genetic enhancement of the ability to tolerate photoinhibition by introduction of unsaturated bonds into membrane glycerolipids [J].
Gombos, Z ;
Kanervo, E ;
Tsvetkova, N ;
Sakamoto, T ;
Aro, EM ;
Murata, N .
PLANT PHYSIOLOGY, 1997, 115 (02) :551-559
[12]  
HANSEN J, 1997, PROSTAGLANDINS LEUKO, V57, P157
[13]   LIPID-COMPOSITION AND BIOSYNTHESIS IN AGING CULTURES OF THE MARINE CRYPTOMONAD, CHROOMONAS-SALINA [J].
HENDERSON, RJ ;
SARGENT, JR .
PHYTOCHEMISTRY, 1989, 28 (05) :1355-1361
[14]   Elucidation of the biosynthesis of eicosapentaenoic acid in the microalga Porphyridium cruentum .2. Studies with radiolabeled precursors [J].
Khozin, I ;
Adlerstein, D ;
Bigongo, C ;
Heimer, YM ;
Cohen, Z .
PLANT PHYSIOLOGY, 1997, 114 (01) :223-230
[15]   Nitrogen starvation induces the accumulation of arachidonic acid in the freshwater green alga Parietochloris incisa (Trebuxiophyceae) [J].
Khozin-Goldberg, I ;
Bigogno, C ;
Shrestha, P ;
Cohen, Z .
JOURNAL OF PHYCOLOGY, 2002, 38 (05) :991-994
[16]   Triacylglycerols of the red microalga Porphyridium cruentum can contribute to the biosynthesis of eukaryotic galactolipids [J].
Khozin-Goldberg, I ;
Yu, HZ ;
Adlerstein, D ;
Didi-Cohen, S ;
Heimer, YM ;
Cohen, Z .
LIPIDS, 2000, 35 (08) :881-889
[17]   Desaturation of fatty acids as an adaptive response to shifts in light intensity [J].
Klyachko-Gurvich, GL ;
Tsoglin, LN ;
Doucha, J ;
Kopetskii, J ;
Ryabykh, IBS ;
Semenenko, VE .
PHYSIOLOGIA PLANTARUM, 1999, 107 (02) :240-249
[18]   ARACHIDONIC-ACID AND EARLY HUMAN GROWTH - IS THERE A RELATION [J].
KOLETZKO, B ;
BRAUN, M .
ANNALS OF NUTRITION AND METABOLISM, 1991, 35 (03) :128-131
[19]  
LICHTENTHALER HK, 1987, METHOD ENZYMOL, V148, P331
[20]   Oleic acid is the main fatty acid related with carotenogenesis in Dunaliella salina [J].
Mendoza, H ;
Martel, A ;
del Río, MJ ;
Reina, GG .
JOURNAL OF APPLIED PHYCOLOGY, 1999, 11 (01) :15-19
123