Molecular analysis of carnivore Protoparvovirus detected in white blood cells of naturally infected cats

被引：22

作者：

Balboni, Andrea ^{[1
]}

Bassi, Francesca ^{[1
]}

De Arcangeli, Stefano ^{[1
]}

Zobba, Rosanna ^{[2
]}

Dedola, Carla ^{[2
]}

Alberti, Alberto ^{[2
]}

Battilani, Mara ^{[1
]}

机构：

[1] Univ Bologna, Dept Vet Med Sci, Alma Mater Studiorum, Via Tolara Sopra 50, I-40064 Ozzano Dell Emilia, BO, Italy

[2] Univ Sassari, Dept Vet Med, Via Vienna 2, I-07100 Sassari, Italy

来源：

BMC VETERINARY RESEARCH | 2018年 / 14卷

关键词：

Canine parvovirus; Cat; Coinfection; Feline panleukopenia virus; White blood cells; PCR; FELINE PANLEUKOPENIA VIRUS; HUMAN PARVOVIRUS B19; CANINE-PARVOVIRUS; GENETIC DIVERSITY; ANTIGENIC TYPES; DOMESTIC CATS; BONE-MARROW; HEMAGGLUTINATION; PERSISTENCE; EMERGENCE;

D O I：

10.1186/s12917-018-1356-9

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Background: Cats are susceptible to feline panleukopenia virus (FPV) and canine parvovirus (CPV) variants 2a, 2b and 2c. Detection of FPV and CPV variants in apparently healthy cats and their persistence in white blood cells (WBC) and other tissues when neutralising antibodies are simultaneously present, suggest that parvovirus may persist long-term in the tissues of cats post-infection without causing clinical signs. The aim of this study was to screen a population of 54 cats from Sardinia (Italy) for the presence of both FPV and CPV DNA within buffy coat samples using polymerase chain reaction (PCR). The DNA viral load, genetic diversity, phylogeny and antibody titres against parvoviruses were investigated in the positive cats. Results: Carnivore protoparvovirus 1 DNA was detected in nine cats (16.7%). Viral DNA was reassembled to FPV in four cats and to CPV (CPV-2b and 2c) in four cats; one subject showed an unusually high genetic complexity with mixed infection involving FPV and CPV-2c. Antibodies against parvovirus were detected in all subjects which tested positive to DNA parvoviruses. Conclusions: The identification of FPV and CPV DNA in the WBC of asymptomatic cats, despite the presence of specific antibodies against parvoviruses, and the high genetic heterogeneity detected in one sample, confirmed the relevant epidemiological role of cats in parvovirus infection.

引用

页数：10

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