Preparation of a murine oral palate wound healing model

被引：1

作者：

Leonardo, Trevor R. ^{[1
,2
]}

Chen, Lin ^{[2
]}

Dipietro, Luisa A. ^{[2
]}

机构：

[1] Univ Illinois, Dept Microbiol & Immunol, Chicago, IL 60612 USA

[2] Univ Illinois, Ctr Wound Healing & Tissue Regenerat, Chicago, IL 60612 USA

来源：

STAR PROTOCOLS | 2022年 / 3卷 / 04期

基金：

美国国家卫生研究院;

关键词：

Health sciences; Model organisms; Tissue engineering;

D O I：

10.1016/j.xpro.2022.101727

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The oral cavity is a highly regenerative epithelial tissue that results in minimal scarring after injury. This protocol describes the preparation of a mouse palate wound model. The protocol includes steps to place an excisional wound on the mouse palate, followed by harvesting of wound tissue and bone decalcification. We detail how to overcome the technical challenge of limited anatomical space, avoid damaging the nasal cavity, manage bleeding, and collect tissue for down-stream genomic or immunohistochemical analysis.

引用

页数：11

共 2 条

[1] Heterogeneity within Stratified Epithelial Stem Cell Populations Maintains the Oral Mucosa in Response to Physiological Stress [J].

Byrd, Kevin M. ;

Piehl, Natalie C. ;

Patel, Jeet H. ;

Huh, Won Jae ;

Sequeira, Ines ;

Lough, Kendall J. ;

Wagner, Bethany L. ;

Marangoni, Pauline ;

Watt, Fiona M. ;

Klein, Ophir D. ;

Coffey, Robert J. ;

Williams, Scott E. .

CELL STEM CELL, 2019, 25 (06) :814-+

[2] IL-1 plays a critical role in oral, put not dermal, wound healing [J].

Graves, DT ;

Nooh, N ;

Gillen, T ;

Davey, M ;

Patel, S ;

Cottrell, D ;

Amar, S .

JOURNAL OF IMMUNOLOGY, 2001, 167 (09) :5316-5320

← 1 →