Functional Analysis of the Trichoderma harzianum nox1 Gene, Encoding an NADPH Oxidase, Relates Production of Reactive Oxygen Species to Specific Biocontrol Activity against Pythium ultimum

被引:54
作者
Montero-Barrientos, M. [1 ]
Hermosa, R. [1 ]
Cardoza, R. E. [2 ]
Gutierrez, S. [2 ]
Monte, E. [1 ]
机构
[1] Univ Salamanca, Spanish Portuguese Ctr Agr Res CIALE, Dept Genet & Microbiol, Salamanca 37185, Spain
[2] Univ Leon, Area Microbiol, Escuela Univ Ciencias Salud, Ponferrada 24400, Spain
关键词
RESPIRATORY BURST OXIDASE; TRICHODERMA-HARZIANUM; OXIDATIVE STRESS; CELL-DEATH; SCLEROTIAL DIFFERENTIATION; HETEROLOGOUS EXPRESSION; ARABIDOPSIS-THALIANA; DISEASE RESISTANCE; PODOSPORA-ANSERINA; BIOLOGICAL-CONTROL;
D O I
10.1128/AEM.02486-10
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The synthesis of reactive oxygen species (ROS) is one of the first events following pathogenic interactions in eukaryotic cells, and NADPH oxidases are involved in the formation of such ROS. The nox1 gene of Trichoderma harzianum was cloned, and its role in antagonism against phytopathogens was analyzed in nox1-overexpressed transformants. The increased levels of nox1 expression in these transformants were accompanied by an increase in ROS production during their direct confrontation with Pythium ultimum. The transformants displayed an increased hydrolytic pattern, as determined by comparing protease, cellulase, and chitinase activities with those for the wild type. In confrontation assays against P. ultimum the nox1-overexpressed transformants were more effective than the wild type, but not in assays against Botrytis cinerea or Rhizoctonia solani. A transcriptomic analysis using a Trichoderma high-density oligonucleotide (HDO) microarray also showed that, compared to gene expression for the interaction of wild-type T. harzianum and P. ultimum, genes related to protease, cellulase, and chitinase activities were differentially upregulated in the interaction of a nox1-overexpressed transformant with this pathogen. Our results show that nox1 is involved in T. harzianum ROS production and antagonism against P. ultimum.
引用
收藏
页码:3009 / 3016
页数:8
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