The IL-3, IL-5, and GM-CSF common receptor beta chain mediates oncogenic activity of FLT3-ITD-positive AML

被引：11

作者：

Charlet, Anne ^{[1
]}

Kappenstein, Max ^{[1
]}

Keye, Philip ^{[2
]}

Klasener, Kathrin ^{[3
,4
]}

Endres, Cornelia ^{[1
,5
]}

Poggio, Teresa ^{[1
,5
,6
]}

Gorantla, Sivahari P. ^{[7
]}

Kreutmair, Stefanie ^{[1
,8
]}

Sanger, Jana ^{[9
]}

Illert, Anna L. ^{[1
,8
]}

Miething, Cornelius ^{[1
,5
,8
]}

Reth, Michael ^{[3
,4
]}

Duyster, Justus ^{[1
,5
,8
]}

Rummelt, Christoph ^{[1
]}

von Bubnoff, Nikolas ^{[1
,7
,8
]}

机构：

[1] Univ Freiburg, Fac Med, Med Ctr, Dept Hematol Oncol & Stem Cell Transplantat, Freiburg, Germany

[2] Univ Freiburg, Fac Med, Eye Ctr, Med Ctr, Freiburg, Germany

[3] Univ Freiburg, Signalling Res Ctr BIOSS & CIBSS, Freiburg, Germany

[4] Univ Freiburg, Fac Biol, Inst Biol 3, Freiburg, Germany

[5] German Canc Res Ctr, Heidelberg, Germany

[6] Albert Ludwigs Univ Freiburg, Fac Biol, D-79106 Freiburg, Germany

[7] Univ Schleswig Holstein, Med Ctr, Dept Hematol & Oncol, Campus Lubeck, Lubeck, Germany

[8] Helmholtz Zentrum Munich, Inst Allergy Res, Munich, Germany

[9] German Canc Consortium DKTK, Partner Site Freiburg, Freiburg, Germany

来源：

LEUKEMIA | 2022年 / 36卷 / 03期

关键词：

ACUTE MYELOID-LEUKEMIA; COLONY-STIMULATING FACTOR; TYROSINE KINASES; FLT3; MUTATIONS; IN-SITU; STAT5; SRC; SUBUNIT; PROTEIN; INTERLEUKIN-3;

D O I：

10.1038/s41375-021-01462-4

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

FLT3-ITD is the most predominant mutation in AML being expressed in about one-third of AML patients and is associated with a poor prognosis. Efforts to better understand FLT3-ITD downstream signaling to possibly improve therapy response are needed. We have previously described FLT3-ITD-dependent phosphorylation of CSF2RB, the common receptor beta chain of IL-3, IL-5, and GM-CSF, and therefore examined its significance for FLT3-ITD-dependent oncogenic signaling and transformation. We discovered that FLT3-ITD directly binds to CSF2RB in AML cell lines and blasts isolated from AML patients. A knockdown of CSF2RB in FLT3-ITD positive AML cell lines as well as in a xenograft model decreased STAT5 phosphorylation, attenuated cell proliferation, and sensitized to FLT3 inhibition. Bone marrow from CSF2RB-deficient mice transfected with FLT3-ITD displayed decreased colony formation capacity and delayed disease onset together with increased survival upon transplantation into lethally irradiated mice. FLT3-ITD-dependent CSF2RB phosphorylation required phosphorylation of the FLT3 juxtamembrane domain at tyrosines 589 or 591, whereas the ITD insertion site and sequence were of no relevance. Our results demonstrate that CSF2RB participates in FLT3-ITD-dependent oncogenic signaling and transformation in vitro and in vivo. Thus, CSF2RB constitutes a rational treatment target in FLT3-ITD-positive AML.

引用

页码：701 / 711

页数：11

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