NRL-Regulated Transcriptome Dynamics of Developing Rod Photoreceptors

被引：88

作者：

Kim, Jung-Woong ^{[1
,2
]}

Yang, Hyun-Jin ^{[1
]}

Brooks, Matthew John ^{[1
]}

Zelinger, Lina ^{[1
]}

Karakulah, Gokhan ^{[1
,7
]}

Gotoh, Norimoto ^{[1
,3
]}

Boleda, Alexis ^{[1
]}

Gieser, Linn ^{[1
]}

Giuste, Felipe ^{[1
]}

Whitaker, Dustin Thad ^{[1
,4
]}

Walton, Ashley ^{[1
]}

Villasmil, Rafael ^{[5
]}

Barb, Jennifer Joanna ^{[6
]}

Munson, Peter Jonathan ^{[6
]}

Kaya, Koray Dogan ^{[1
]}

Chaitankar, Vijender ^{[1
]}

Cogliati, Tiziana ^{[1
]}

Swaroop, Anand ^{[1
]}

机构：

[1] NEI, Neurobiol Neurodegenerat & Repair Lab, NIH, Bethesda, MD 20892 USA

[2] Chung Ang Univ, Dept Life Sci, Seoul 06974, South Korea

[3] Kyoto Univ, Grad Sch Med, Ctr Genom Med, Kyoto 6068501, Japan

[4] Texas A&M Univ, Texas A&M Inst Neurosci, College Stn, TX 77843 USA

[5] NEI, Flow Cytometry Core, NIH, Bethesda, MD 20892 USA

[6] NIH, Math & Stat Comp Lab, Ctr Informat Technol, Bldg 10, Bethesda, MD 20892 USA

[7] Dokuz Eylul Univ, Izmir Int Biomed & Genome Inst, TR-35340 Inciralti, Turkey

来源：

CELL REPORTS | 2016年 / 17卷 / 09期

基金：

新加坡国家研究基金会;

关键词：

NUCLEAR RECEPTOR NR2E3; MOUSE RETINA; LEUCINE-ZIPPER; BIPOLAR CELLS; IN-VIVO; EXPRESSION; DIFFERENTIATION; BINDING; PROTEIN; CRX;

D O I：

10.1016/j.celrep.2016.10.074

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Gene regulatory networks (GRNs) guiding differentiation of cell types and cell assemblies in the nervous system are poorly understood because of inherent complexities and interdependence of signaling pathways. Here, we report transcriptome dynamics of differentiating rod photoreceptors in the mammalian retina. Given that the transcription factor NRL determines rod cell fate, we performed expression profiling of developing NRL-positive (rods) and NRL-negative (S-cone-like) mouse photoreceptors. We identified a large-scale, sharp transition in the transcriptome landscape between postnatal days 6 and 10 concordant with rod morphogenesis. Rod-specific temporal DNA methylation corroborated gene expression patterns. De novo assembly and alternative splicing analyses revealed previously unannotated rod-enriched transcripts and the role of NRL in transcript maturation. Furthermore, we defined the relationship of NRL with other transcriptional regulators and downstream cognate effectors. Our studies provide the framework for comprehensive system-level analysis of the GRN underlying the development of a single sensory neuron, the rod photoreceptor.

引用

页码：2460 / 2473

页数：14

共 72 条

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