In vitro expansion and differentiation of fresh and revitalized adult canine bone marrow-derived and adipose tissue-derived stromal cells

被引：33
作者：
Spencer, Nakia D. [1 ]
Chun, Raymond [1 ]
Vidal, Martin A. [1 ]
Gimble, Jeffrey M. [2 ]
Lopez, Mandi J. [1 ]
机构：
[1] Louisiana State Univ, Sch Vet Med, Lab Equine & Comparat Orthoped Res, Baton Rouge, LA 70803 USA
[2] Pennington Biomed Res Ctr, Stem Cell Biol Lab, Baton Rouge, LA 70808 USA
来源：
VETERINARY JOURNAL | 2012年 / 191卷 / 02期
基金：
美国国家卫生研究院;
关键词：
Canine; Multi-potent stromal cells; Bone; Adipose tissue; Cryopreservation; MESENCHYMAL STEM-CELLS; OSTEOGENIC CAPACITY; FREQUENCY; FAT; OSTEOARTHRITIS; COMPONENTS; CARTILAGE; DISEASE; CULTURE; LINE;
D O I：
10.1016/j.tvjl.2010.12.030
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
The objective of this study was to determine the tissue density, in vitro expansion and differentiation of canine adipose tissue-derived (ASC) and bone marrow-derived (BMSC) stromal cells. Primary (PO) and cell passages 1-6 (P1-6) cell doubling numbers (CD) and doubling times (DT) were determined in fresh cells. The P0, P3, and P6 adipogenic (CFU-Ad), osteogenic (CFU-Ob), and fibroblastic (CFU-F) colony forming unit frequencies, lineage specific mRNA levels in differentiated P3 cells and composition of P3 and P6 chondrogenic pellets were assessed in cryogenically preserved cells. Cell yields from bone marrow were significantly higher than adipose tissue. Overall ASC and BMSC CDs and DTs and P3 and P6 CFU-F, CFU-Ad, and CFU-Ob were comparable. The P0 BMSC CFU-Ob was significantly higher than ASC. Lineage specific mRNA levels were higher in differentiated versus control cells, but similar between cell types. Protein was significantly greater in P3 versus P6 ASC chondrogenic pellets. Based on these findings, fresh and revitalized canine ASCs are viable alternatives to BMSCs for stromal cell applications. Published by Elsevier Ltd.
引用
收藏
页码：231 / 239
页数：9
相关论文
共 36 条
[1]   Isolation, characterization and osteogenic differentiation of adipose-derived stem cells: from small to large animal models [J].
Arrigoni, Elena ;
Lopa, Silvia ;
de Girolamo, Laura ;
Stanco, Deborah ;
Brini, Anna T. .
CELL AND TISSUE RESEARCH, 2009, 338 (03) :401-411
[2]   Yield of human adipose-derived adult stem cells from liposuction aspirates [J].
Aust, L ;
Devlin, B ;
Foster, SJ ;
Halvorsen, YDC ;
Hicok, K ;
du Laney, T ;
Sen, A ;
Willingmyre, GD ;
Gimble, JM .
CYTOTHERAPY, 2004, 6 (01) :7-14
[3]   Preadipocyte number in omental and subcutaneous adipose tissue of obese individuals [J].
Bakker, AHF ;
van Dielen, FMH ;
Greve, JWM ;
Adam, JA ;
Buurman, WA .
OBESITY RESEARCH, 2004, 12 (03) :488-498
[4]  
Black L, 2008, VET THER, V9
[5]  
Black LL, 2007, VET THER, V8, P272
[6]   Bone marrow mesenchymal stromal cells (BM-MSCs) from healthy donors and auto-immune disease patients reduce the proliferation of autologous- and allogeneic-stimulated lymphocytes in vitro [J].
Bocelli-Tyndall, C. ;
Bracci, L. ;
Spagnoli, G. ;
Braccini, A. ;
Bouchenaki, M. ;
Ceredig, R. ;
Pistoia, V. ;
Martin, I. ;
Tyndall, A. .
RHEUMATOLOGY, 2007, 46 (03) :403-408
[7]   Mesenchymal stem cells: Cell-based reconstructive therapy in orthopedics [J].
Caplan, AI .
TISSUE ENGINEERING, 2005, 11 (7-8) :1198-1211
[8]   Propagation and senescence of human marrow stromal cells in culture: a simple colony-forming assay identifies samples with the greatest potential to propagate and differentiate [J].
DiGirolamo, CM ;
Stokes, D ;
Colter, D ;
Phinney, DG ;
Class, R ;
Prockop, DJ .
BRITISH JOURNAL OF HAEMATOLOGY, 1999, 107 (02) :275-281
[9]   A new preadipocyte cell line, AP-18, established from adult mouse adipose tissue [J].
Doi, H ;
Masaki, N ;
Takahashi, H ;
Komatsu, H ;
Fujimori, K ;
Satomi, S .
TOHOKU JOURNAL OF EXPERIMENTAL MEDICINE, 2005, 207 (03) :209-216
[10]   Tissue-engineered cartilage and bone using stem cells from human infrapatellar fat pads [J].
Dragoo, JL ;
Samimi, B ;
Zhu, M ;
Hame, SL ;
Thomas, BJ ;
Lieberman, JR ;
Hedrick, MH ;
Benhaim, P .
JOURNAL OF BONE AND JOINT SURGERY-BRITISH VOLUME, 2003, 85B (05) :740-747
1234