Bioprospecting Portuguese Atlantic coast cyanobacteria for bioactive secondary metabolites reveals untapped chemodiversity

被引:30
作者
Brito, Angela [1 ,2 ,3 ]
Gaifem, Joana [1 ,2 ]
Ramos, Vitor [4 ]
Glukhov, Evgenia [5 ]
Dorrestein, Pieter C. [6 ,7 ]
Gerwick, William H. [5 ,7 ]
Vasconcelos, Vitor M. [3 ,4 ]
Mendes, Marta V. [1 ,2 ]
Tamagnini, Paula [1 ,2 ,3 ]
机构
[1] Univ Porto, Inst Invest Inovacao Saude i3S, P-4150180 Oporto, Portugal
[2] Univ Porto, IBMC, P-4150180 Oporto, Portugal
[3] Univ Porto, Dept Biol, Fac Ciencias, P-4150180 Oporto, Portugal
[4] Univ Porto, CIIMAR CIMAR Interdisciplinary Ctr Marine & Envir, P-4150180 Oporto, Portugal
[5] Univ Calif San Diego, Scripps Inst Oceanog, Ctr Marine Biotechnol & Biomed, La Jolla, CA 92093 USA
[6] Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USA
[7] Univ Calif San Diego, Skaggs Sch Pharm & Pharmaceut Sci, La Jolla, CA 92093 USA
来源
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS | 2015年 / 9卷
关键词
Atlantic Ocean; Bioactive compounds; Marine cyanobacteria; Molecular networking; NRPS; PKS; PEPTIDE SYNTHETASE GENES; MARINE CYANOBACTERIUM; MOLECULAR NETWORKING; CYCLIC DEPSIPEPTIDES; POLYKETIDE SYNTHASE; LYNGBYA-MAJUSCULA; DIVERSITY; BIOSYNTHESIS; TOXICITY; CULTURES;
D O I
10.1016/j.algal.2015.03.016
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Continental Portugal has an extensive Atlantic Ocean coastline; however little is known about the diversity of its marine cyanobacteria, with only a few reports published. Cyanobacteria are a prolific source of bioactive compounds with promising therapeutic applications. Previously, several cyanobacterial strains (Subsections I-IV) were isolated from the Portuguese Atlantic coast and characterized using a polyphasic approach. Preliminary screening indicated that these cyanobacteria lacked the genes for proteins involved in the production of conventional cyanotoxins. However, it was previously shown that extracts of marine Synechocystis and Synechococcus were toxic to invertebrates, with crude extracts causing stronger effects than partially purified ones. To further evaluate the potential of our temperate region Portuguese isolates to produce bioactive compounds, a PCR screening for the presence of genes encoding non-ribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs), targeting the adenylation (A) and ketosynthase (KS) domains respectively, was performed. DNA fragments were obtained for more than 80% of the strains tested, and the results revealed that PKS genes are more ubiquitous than NRPS genes. The sequences obtained were used in an in silico prediction of the PKS and NRPS systems. RT-PCR analyses revealed that these genes are transcribed under routine laboratory conditions in several selected strains. Furthermore, LC-MS analysis coupled with molecular networking, a mass spectrometric tool that clusters metabolites with similarMS/MS fragmentation patterns, was used to search for novel or otherwise interesting metabolites revealing an untapped chemodiversity. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码:218 / 226
页数:9
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