Campylobacter from sows in farrow-to-finish pig farms: Risk indicators and genetic diversity

被引:17
作者
Denis, Martine [1 ]
Henrique, Emmanuelle [1 ]
Chidaine, Berengere [1 ]
Tircot, Annie [1 ]
Bougeard, Stephanie [2 ]
Fravalo, Philippe [1 ]
机构
[1] Anses, Lab Ploufragan Plouzane, Unite Hyg & Quante Prod Avicoles & Porcins, F-22440 Ploufragan, France
[2] Unite Epidemiol & Bien Etre Porc, F-22440 Ploufragan, France
关键词
Campylobacter; Sows; Fattening pig; Risk indicators; PFGE; FATTENING PIGS; TYPING METHODS; COLI; PREVALENCE; JEJUNI; SPP; INFECTIONS; FRANCE; COLONIZATION; PROFILES;
D O I
10.1016/j.vetmic.2011.07.001
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Sows have been identified as a source of Campylobacter contamination in piglets. We carried out a one-year study, in 2008, at 53 farrow-to-finish farms in Brittany, France, to determine the proportion of sows excreting Campylobacter. We also determined the genotypes of the Campylobacter isolates. Moreover, Generalized Estimating Equations including repeated effects were used to assess the association between management practices and farm characteristics, and risk of Campylobacter shedding by sows. Per farm, 10 feces samples from sows were collected from selected sites (maternity, service area, gestation area) on the farms. Campylobacter isolates were identified by PCR and typed by PFGE. Campylobacter was detected in 25.1% of the 530 samples from sows, and 67% of the 53 pig farms had at least one positive sample (of 10 taken). All the Campylobacter isolates belonged to the Campylobacter coil species. They displayed a very high level of genetic diversity, also inside farms and few genotypes were common to several farms. Warmer months, large farms, and individual housing for sows were identified as risk indicators of Campylobacter shedding by sows. A short delay between sampling and treatment of the samples should be considered, to improve the detection of the bacterium in the feces samples. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:163 / 170
页数:8
相关论文
共 33 条
[1]  
Buswell CM, 1998, APPL ENVIRON MICROB, V64, P733
[2]   Diversity of pulsed-field gel electrophoresis profiles of Campylobacter jejuni and Campylobacter coli from broiler chickens in France [J].
Denis, M. ;
Rose, V. ;
Huneau-Salaun, A. ;
Balaine, L. ;
Salvat, G. .
POULTRY SCIENCE, 2008, 87 (08) :1662-1671
[3]   Comparison of genetic profiles of Campylobacter strains isolated from poultry, pig and Campylobacter human infections in Brittany, France [J].
Denis, M. ;
Chidaine, B. ;
Laisney, M-J. ;
Kempf, I. ;
Rivoal, K. ;
Megraud, F. ;
Fravalo, P. .
PATHOLOGIE BIOLOGIE, 2009, 57 (01) :23-29
[4]  
*EFSA, 2009, EFSA J, V157
[5]   Foodborne zoonoses due to meat: a quantitative approach for a comparative risk assessment applied to pig slaughtering in Europe [J].
Fosse, Julien ;
Seegers, Henri ;
Magras, Catherine .
VETERINARY RESEARCH, 2008, 39 (01)
[6]   Risk factors for sporadic Campylobacter infection in the United States:: A case-control study in FoodNet sites [J].
Friedman, CR ;
Hoekstra, RM ;
Samuel, M ;
Marcus, R ;
Bender, J ;
Shiferaw, B ;
Reddy, S ;
Ahuja, SD ;
Helfrick, DL ;
Hardnett, F ;
Carter, M ;
Anderson, B ;
Tauxe, RV .
CLINICAL INFECTIOUS DISEASES, 2004, 38 :S285-S296
[7]   A case-case comparison of Campylobacter coli and Campylobacter jejuni infection:: A tool for generating hypotheses [J].
Gillespie, IA ;
O'Brien, SJ ;
Frost, JA ;
Adak, GK ;
Horby, P ;
Swan, AV ;
Painter, MJ ;
Neal, KR .
EMERGING INFECTIOUS DISEASES, 2002, 8 (09) :937-942
[8]   Determining confidence intervals when measuring genetic diversity and the discriminatory abilities of typing methods for microorganisms [J].
Grundmann, H ;
Hori, S ;
Tanner, G .
JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (11) :4190-4192
[9]  
HUNEAUSALATIN A, 2007, VET MED, V80, P34