Adhesion force sensing and activation of a membrane-bound sensor to activate nisin efflux pumps in Staphylococcus aureus under mechanical and chemical stresses

被引:23
作者
Carniello, Vera
Harapanahalli, Akshay K.
Busscher, Henk J.
van der Mei, Henny C.
机构
[1] Univ Groningen, Univ Med Ctr Groningen, Groningen, Netherlands
[2] Univ Groningen, Univ Med Ctr Groningen, Dept Biomed Engn, Groningen, Netherlands
关键词
Nisin; NsaRS response-regulator; Antibiotic susceptibility; Biofilms; Cell wall deformation; Surface sensing; Adhesion force; Atomic force microscopy; Gene expression; BACTERIAL ADHESION; GENE-EXPRESSION; SURFACES; SUSCEPTIBILITY; TRANSDUCTION; SPECTROSCOPY; EPIDERMIDIS; PROTEINS; BIOFILMS; SYSTEMS;
D O I
10.1016/j.jcis.2017.10.024
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Nisin-associated-sensitivity-response-regulator (NsaRS) in Staphylococcus aureus is important for its adhesion to surfaces and resistance against antibiotics, like nisin. NsaRS consists of an intra-membrane-located sensor NsaS and a cytoplasmatically-located response-regulator NsaR, which becomes activated upon receiving phosphate groups from the intratmembrane-located sensor. Hypothesis: The intra-membrane location of the NsaS sensor leads us to hypothesize that the two component NsaRS system not only senses "chemical" (nisin) but also "mechanical" (adhesion) stresses to modulate efflux of antibiotics from the cytoplasm. Experiments: NsaS sensor and NsaAB efflux pump transcript levels in S. aureus SH1000 adhering to surfaces exerting different adhesion forces were compared, in presence and absence of nisin. Adhesion forces were measured using single-bacterial contact probe atomic force microscopy. Findings: Gene expression became largest when staphylococci experienced strong adhesion forces combined with nisin-presence and the two-component NsaRS response to antibiotics was enhanced at a stronger adhesion force. This confirms that the intra-membrane-located sensor NsaS senses both chemical and mechanical stresses to modulate antibiotic clearance through the NsaAB efflux pump. This finding creates better understanding of the antibiotic resistance of bacteria adhering to surfaces and, in the fight against antibiotic-resistant pathogens, may aid development of advanced biomaterials on which bacterial efflux pumps are not activated. (C) 2017 Elsevier Inc. All rights reserved.
引用
收藏
页码:14 / 20
页数:7
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