A Simple Method for DNA Extraction from Activated Sludge

被引：0
|
作者：
Singka, Dounghatai [1 ]
Kumdhitiahutsawakul, Ladapa [2 ]
Rekkriangkrai, Prasert [2 ]
Pathom-aree, Wasu [1 ]
机构：
[1] Chiang Mai Univ, Dept Biol, Fac Sci, Chiang Mai 50200, Thailand
[2] Chiang Mai Univ, Energy Res & Dev Inst Nakornping, Chiang Mai 50200, Thailand
来源：
CHIANG MAI JOURNAL OF SCIENCE | 2012年 / 39卷 / 01期
关键词：
Activated sludge; DNA extraction; DGGE; Archaeal population; GRADIENT GEL-ELECTROPHORESIS; WASTE-WATER TREATMENT; ENVIRONMENTAL DNA; PURIFICATION; SOIL;
D O I：
暂无
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A protocol for DNA extraction directly from activated sludge is described. Sludge samples were lysed by glass bead and CTAB. The extracted DNA was purified with phenol: chloroform: isoamyl alcohol mixture and precipitated in sodium acetate and isopropanol. The proposed protocol is simple, rapid and required small sample size, yielding about 40.5 +/- 1.8 mu g DNA g(-1) sludge. The quality of DNA is good enough for successful PCR amplifications of 16S rDNA, 16S rDNA V3 region and denaturing gradient gel electrophoresis (DGGE) analysis of archaeal population. This described method can also be applied to other environmental samples such as whey and soil.
引用
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页码：111 / 118
页数：8
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