Grazing-incidence small-angle X-ray scattering from alkaline phosphatase immobilized in atmospheric plasmapolymer coatings

被引：7

作者：

Ortore, M. G. ^{[1
]}

Sinibaldi, R. ^{[1
]}

Heyse, P. ^{[2
,3
]}

Paulussen, S. ^{[2
]}

Bernstorff, S. ^{[4
]}

Sels, B.

Mariani, P. ^{[1
]}

Rustichelli, F. ^{[1
]}

Spinozzi, F. ^{[1
]}

机构：

[1] Univ Politecn Marche, Dipartimento SASC, I-60131 Ancona, Italy

[2] VITO Mat Technol, Boeretang, Belgium

[3] Katholieke Univ Leuven, Ctr Surface Chem & Catalysis, B-3001 Heverlee, Belgium

[4] Sincrotrone Trieste, I-34012 Trieste, Italy

来源：

APPLIED SURFACE SCIENCE | 2008年 / 254卷 / 17期

关键词：

GISAXS; polymer coatings; proteins; atmospheric-pressure plasma deposition;

D O I：

10.1016/j.apsusc.2008.02.118

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Grazing-incidence small-angle X-ray scattering (GISAXS) has been used to study proteins embedded in thin polymer films obtained by a new cold, atmospheric-pressure plasma technique. In order to test the efficiency of the technology, four samples of alkaline phosphatase incorporated in organic polymer coatings in different plasma conditions have been investigated. Data have been analysed in the framework of the distorted-wave Born approximation (DWBA), by using a new method for the simultaneous fitting of the two-dimensional diffuse scattering from each sample. As a result, protein film concentration and aggregation state as well as a set of parameters describing the polymer coatings have been obtained. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：5557 / 5563

页数：7

共 24 条

[21]

2-5

[22] Interaction of proteins in solution from small-angle scattering: A perturbative approach [J].

Spinozzi, F ;

Gazzillo, D ;

Giacometti, A ;

Mariani, P ;

Carsughi, F .

BIOPHYSICAL JOURNAL, 2002, 82 (04) :2165-2175

[23]

Wang J, 2002, PHYS REV B, V66, DOI 10.1103/PhysRevB.66.161201

[24] Characterization of a highly thermostable alkaline phosphatase from the euryarchaeon Pyrococcus abyssi [J].

Zappa, S ;

Rolland, JL ;

Flament, D ;

Gueguen, Y ;

Boudrant, J ;

Dietrich, J .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (10) :4504-4511

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