Phenotypic and Genotypic Characterization of Biofilm Formation in Staphylococcus haemolyticus

被引:25
作者
Barros, Elaine M. [1 ]
Lemos, Moara [2 ]
Souto-Padron, Thais [2 ]
Giambiagi-deMarval, Marcia [1 ]
机构
[1] Univ Fed Rio de Janeiro, Inst Microbiol Paulo de Goes, Dept Microbiol Med, BR-21941902 Rio De Janeiro, Brazil
[2] Univ Fed Rio de Janeiro, Inst Microbiol Paulo de Goes, Dept Microbiol Geral, BR-21941902 Rio De Janeiro, Brazil
关键词
BAP; EPIDERMIDIS; AUREUS; MECHANISMS; STRAINS; ICA;
D O I
10.1007/s00284-015-0794-x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Staphylococcus haemolyticus is one of the most frequently isolated coagulase-negative staphylococci. The ability to produce biofilm has contributed to its emergence as a nosocomial pathogen. In this study, some growth conditions were tested to determine their influence on biofilm formation. Brain-heart infusion (BHI) broth containing glucose was used to screen 64 clinical strains. A strong biofilm producer strain showed cells surrounded by a thick layer of extracellular matrix. The presence of atlE, fbp, bap, and icaA genes was analyzed. We concluded that S. haemolyticus biofilm production can be increased with cells grown in BHI, and highlighted that it could be an ica-independent process.
引用
收藏
页码:829 / 834
页数:6
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