Phenotypic and Genotypic Characterization of Biofilm Formation in Staphylococcus haemolyticus

被引：26

作者：

Barros, Elaine M. ^{[1
]}

Lemos, Moara ^{[2
]}

Souto-Padron, Thais ^{[2
]}

Giambiagi-deMarval, Marcia ^{[1
]}

机构：

[1] Univ Fed Rio de Janeiro, Inst Microbiol Paulo de Goes, Dept Microbiol Med, BR-21941902 Rio De Janeiro, Brazil

[2] Univ Fed Rio de Janeiro, Inst Microbiol Paulo de Goes, Dept Microbiol Geral, BR-21941902 Rio De Janeiro, Brazil

来源：

CURRENT MICROBIOLOGY | 2015年 / 70卷 / 06期

关键词：

BAP; EPIDERMIDIS; AUREUS; MECHANISMS; STRAINS; ICA;

D O I：

10.1007/s00284-015-0794-x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Staphylococcus haemolyticus is one of the most frequently isolated coagulase-negative staphylococci. The ability to produce biofilm has contributed to its emergence as a nosocomial pathogen. In this study, some growth conditions were tested to determine their influence on biofilm formation. Brain-heart infusion (BHI) broth containing glucose was used to screen 64 clinical strains. A strong biofilm producer strain showed cells surrounded by a thick layer of extracellular matrix. The presence of atlE, fbp, bap, and icaA genes was analyzed. We concluded that S. haemolyticus biofilm production can be increased with cells grown in BHI, and highlighted that it could be an ica-independent process.

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收藏

页码：829 / 834

页数：6

相关论文

共 22 条

[1] Presence of icaA and icaD genes and slime production in a collection of staphylococcal strains from catheter-associated infections [J].

Arciola, CR ;

Baldassarri, L ;

Montanaro, L .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (06) :2151-2156

[2] Calcium inhibits bap-dependent multicellular behavior in Staphylococcus aureus [J].

Arrizubieta, MJ ;

Toledo-Arana, A ;

Amorena, B ;

Penadés, JR ;

Lasa, I .

JOURNAL OF BACTERIOLOGY, 2004, 186 (22) :7490-7498

[3]

Atshan SS, 2012, J BIOMED BIOTECHNOL, V2012, P1, DOI DOI 10.1155/2012/417247

[4] Staphylococcus epidermidis Biofilm Formation and Structural Organization on Different Types of Intraocular Lenses under in vitro Flow Conditions [J].

Baillif, Stephanie ;

LeDuff, Frank ;

Hartmann, Daniel J. ;

Kodjikian, Laurent .

OPHTHALMIC RESEARCH, 2013, 50 (02) :83-90

[5] Staphylococcus haemolyticus as an Important Hospital Pathogen and Carrier of Methicillin Resistance Genes [J].

Barros, E. M. ;

Ceotto, H. ;

Bastos, M. C. F. ;

dos Santos, K. R. N. ;

Giambiagi-deMarval, M. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2012, 50 (01) :166-168

[6] Coagulase-Negative Staphylococci [J].

Becker, Karsten ;

Heilmann, Christine ;

Peters, Georg .

CLINICAL MICROBIOLOGY REVIEWS, 2014, 27 (04) :870-926

[7] Bap, a Staphylococcus aureus surface protein involved in biofilm formation [J].

Cucarella, C ;

Solano, C ;

Valle, J ;

Amorena, B ;

Lasa, I ;

Penadés, JR .

JOURNAL OF BACTERIOLOGY, 2001, 183 (09) :2888-2896

[8] Characterization of methicillin-resistant Staphylococcus aureus isolates from Portuguese hospitals by multiple genotyping methods [J].

deSousa, MA ;

Sanches, IS ;

vanBelkum, A ;

vanLeeuwen, W ;

Verbrugh, H ;

deLencastre, H .

MICROBIAL DRUG RESISTANCE-MECHANISMS EPIDEMIOLOGY AND DISEASE, 1996, 2 (03) :331-341

[9]

Eidhin DN, 1998, MOL MICROBIOL, V30, P245

[10] Clonality of slime-producing methicillin-resistant coagulase-negative staphylococci disseminated in the neonatal intensive care unit of a university hospital [J].

Foka, A. ;

Chini, V. ;

Petinaki, E. ;

Kolonitsiou, F. ;

Anastassiou, E. D. ;

Dimitracopoulos, G. ;

Spiliopoulou, I. .

CLINICAL MICROBIOLOGY AND INFECTION, 2006, 12 (12) :1230-1233