Establishment of poly(ADP-ribose) polymerase-deficient mouse embryonic stem cell lines

被引：12
作者：
Masutani, M [1 ]
Nozaki, T
Nishiyama, E
Ochiya, T
Nakagama, H
Wakabayashi, K
Suzuki, H
Sugimura, T
机构：
[1] Natl Canc Ctr, Res Inst, Div Biochem, Chuo Ku, Tokyo 1040045, Japan
[2] Natl Canc Ctr, Res Inst, Sect Studies Metastasis, Chuo Ku, Tokyo 1040045, Japan
[3] Natl Canc Ctr, Res Inst, Can Prevent Div, Chuo Ku, Tokyo 1040045, Japan
[4] Chugai Pharmaceut Co Ltd, Shizuoka 4120038, Japan
来源：
PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES | 1998年 / 74卷 / 10期
关键词：
Gene-targeting; poly(ADP-ribose) polymerase; embryonic stem cells;
D O I：
10.2183/pjab.74.233
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Many studies revealed that poly(ADP-ribose) polymerase (Parp) is involved in DNA-damage recovery, cell-death induction and maintenance of genomic stability. We generated mouse Parp(+/-) embryonic stem (ES) cell lines by disrupting one allele of Parp exon 1 with a neomycin-resistance gene-cassette and subsequently produced Parp(-/-) ES cells by disrupting the remaining allele with a puromycin-resistance gene-cassette. Parp activity was decreased to half in Parp(+/-) ES cell clones and lost in Parp(-/-) ES cell clones. Growth rates of Parp(+/-) and Parp(-/-) ES cell clones were similar to that of parental J1 ES cells, indicating that Parp is not required for ES cell proliferation. These Parp(-/-) ES cells will be useful to study biological role of poly(ADP-ribosyl)ation reaction.
引用
收藏
页码：233 / 236
页数：4
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