Inflammation inhibits GPR81 expression in adipose tissue

被引：27

作者：

Feingold, Kenneth R. ^{[1
]}

Moser, Arthur ^{[1
]}

Shigenaga, Judy K. ^{[1
]}

Grunfeld, Carl ^{[1
]}

机构：

[1] Univ Calif San Francisco, Metab Sect 111F, Dept Vet Affairs, Med Ctr, San Francisco, CA 94121 USA

来源：

INFLAMMATION RESEARCH | 2011年 / 60卷 / 10期

关键词：

LPS; Zymosan; Turpentine; Cytokine; Adipose; NECROSIS-FACTOR-ALPHA; 3T3-L1; ADIPOCYTES; LACTIC ACID; PPAR-GAMMA; FAT-CELLS; LIPOLYSIS; LACTATE; METABOLISM; KINASE; MECHANISMS;

D O I：

10.1007/s00011-011-0361-2

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Objective and design The aim of this study was to examine the expression of G protein-coupled receptor 81 (GPR81) in mouse adipose tissue in response to inflammatory stimuli. GPR81 is activated by lactate resulting in the inhibition of lipolysis. Materials and treatment Mice were injected with saline, lipopolysaccharide (LPS), zymosan, or turpentine, N = 5 per group. 3T3-L1 adipocytes were treated with tumor necrosis factor alpha, interleukin (IL)-1 beta, IL-6, or interferon gamma. Methods GPR81 expression levels were measured by real-time PCR and statistical significance was determined by Student's t test. Results LPS resulted in a marked decrease in GPR81 mRNA level in mouse adipose tissue in C57BL/6 and OuJ mice, an effect that was not observed in HeJ mice, which have a mutation in TLR4. Zymosan and turpentine also decreased adipose tissue GPR81expression. Cytokine treatment of 3T3-L1 adipocytes had no effect on GPR81 expression. GPR81 expression was decreased in ob/ob mice, an animal model of type 2 diabetes that is characterized by inflammation. Conclusion Inflammation decreases the expression of GPR81 in adipose tissue.

引用

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页码：991 / 995

页数：5

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