Hoogsteen base-pairing in DNA replication? Reply

被引：3

作者：

Aggarwal, A ^{[1
]}

Nair, D

Johnson, R

Prakash, L

Prakash, S

机构：

[1] Mt Sinai Sch Med, Dept Physiol & Biophys, New York, NY 10029 USA

[2] Univ Texas, Med Branch, Sealy Ctr Mol Sci, Galveston, TX 77755 USA

来源：

NATURE | 2005年 / 437卷 / 7057期

关键词：

D O I：

10.1038/nature04200

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have solved the crystal structure of human DNA polymerase-? (hPol?) bound to a template primer and incoming nucleotide, and shown that hPol? uses Hoogsteen base-pairing for normal DNA synthesis. Jimin Wang questions the space group we used and claims that Hoogsteen base-pairing is not supported by our electron-density maps. We show here that the issue of space group does not affect our conclusions and that our electron-density maps are clear in indicating that Hoogsteen and not Watson-Crick base-pairing occurs in this situation. © 2005 by Nature Publishing Group.

引用

页码：E7 / E7

页数：1

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