School enterprise cooperation, the talents training mode, not only meets the needs of China's economic and social development, but also suits talent training in higher education institutions. Therefore, it is an effective teaching method for local universities to realize the cultivation of applied talents. This paper, based on the school enterprise cooperation, studies the training model of the applied talents in local universities database. The article discusses how to build talent training program of school enterprise cooperation; and under the new teaching model, the reform of the database course construction, teaching methods, and the teaching group. In this way, we can achieve a clear understanding of student learning objectives, mobilize the students, and cultivate students' work ability and professional quality, which has certain reference significance for the training of database application talents.