BIOSURFACTANT PRODUCTION BY Pseudomonas sp. ISOLATED FROM HOSPITAL CLINICAL SAMPLES

被引：0

作者：

Poyil, Muhammad Musthafa ^{[1
]}

Palsamy, Sasikumar ^{[2
]}

Karuppiah, Ponmurugan ^{[3
]}

机构：

[1] Prince Sattam Bin Abdul Aziz Univ, Coll Med, Dept Basic Med Sci, Al Kharj 11942, Saudi Arabia

[2] Madura Coll, Dept Microbiol, Madurai, Tamil Nadu, India

[3] King Saud Univ, Coll Sci, Dept Bot & Microbiol, P Box 2455, Riyadh 11451, Saudi Arabia

来源：

APPLIED BIOLOGICAL RESEARCH | 2021年 / 23卷 / 03期

关键词：

Bioremediation; biosurfactant; crude oil degradation; Pseudomonas; OIL;

D O I：

10.5958/0974-4517.2021.00035.5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Biosurfactants are amphiphilic bioactive compounds synthesized extracellularly by various microbes from various substrates like sugar, oil and other waste materials. In present study a biosurfactant producing bacterium was isolated from clinical samples and characterized morphobiochemically. The isolated bacterium was tentatively identified as Pseudomonas sp. and evaluated for its oil degrading ability. The growth and biomass production by the bacterium was optimized using crude oil at different concentrations at variable incubation periods. The maximum growth was observed after 8 days of incubation on minimal medium containing 20% crude oil while maximum biomass (0.1 g) was noticed after 10 days incubation on basal medium containing 25% crude oil. Based on Rf value in TLC, the biosurfactant produced was of rhaminolipid type I with Rf value 0.736. The isolated bacterium can be employed in bioremediation processes.

引用

页码：298 / 302

页数：5

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