Expression of bone morphogenetic protein 15 (BMP-15) during In vitro maturation in canine oocytes

被引：0
作者：
Andres Castro-Sanchez, Rodrigo [1 ,2 ]
Alfredo Palomino-McKenney, Jaime [1 ,2 ]
机构：
[1] Univ Chile, Fac Ciencias Vet & Pecuarias, Lab Reprod Anim, Santiago, Chile
[2] CONICYT, Santiago, Region Metropol, Chile
来源：
REVISTA CIENTIFICA-FACULTAD DE CIENCIAS VETERINARIAS | 2016年 / 26卷 / 04期
关键词：
BMP-15; canine; cumulus cells; oocyte maturation; GROWTH-DIFFERENTIATION FACTOR-9; SYNERGISTIC ROLES; OVARIAN-FOLLICLES; GENE-EXPRESSION; OVULATION RATE; CELL-FUNCTION; BMP15; MOUSE; GDF9; GROWTH-DIFFERENTIATION-FACTOR-9;
D O I：
暂无
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
This research aims to characterize the pattern of expression of BMP-15 during in vitro maturation of canine oocyte. BMP-15 is a follicular factor related to the oocyte maturation, taking part in cumulus cells expansion and resumption meiotic, however still has not been described in the bitch, whose cumulus expansion and meiotic resumption occurs with delay, hindering the replication in labs condition, limiting the success rate in the in vitro maturation of oocytes. BMP-15 is a possible responsible. By mean of western blotting and indirect immunofluorescence, BMP-15 was evaluated in canine cumulus oocyte complex (COCs) cultured during 48, 72 and 96 hours. BMP-15 is present both oocyte and cumulus cells increased its level significantly (P<0.05) while in vitro culture progress. BMP-15 is related whit meiotic progression and cumulus cells expansion. So, BMP-15 has a role in the oocyte maturation of the bitch.
引用
收藏
页码：220 / 226
页数：7
相关论文
共 29 条
  • [1] Sperm Nuclear Decondensation Induction Capacity of In Vitro and In Vivo Matured Canine Oocytes
    De los Reyes, M.
    Palomino, J.
    Parraguez, V. H.
    Vergara, J.
    [J]. REPRODUCTION IN DOMESTIC ANIMALS, 2012, 47 : 98 - 101
  • [2] Mitochondrial distribution and meiotic progression in canine oocytes during in vivo and in vitro maturation
    De los Reyes, M.
    Palomino, J.
    Parraguez, V. H.
    Hidalgo, M.
    Saffie, P.
    [J]. THERIOGENOLOGY, 2011, 75 (02) : 346 - 353
  • [3] Expression of growth differentiation factor 9 (GDF-9) during in vitro maturation in canine oocytes
    De los Reyes, Monica
    Rojas, Claudia
    Hugo Parraguez, Victor
    Palomino, Jaime
    [J]. THERIOGENOLOGY, 2013, 80 (06) : 587 - 596
  • [4] Mutations in an oocyte-derived growth factor gene (BMP15) cause increased ovulation rate and infertility in a dosage-sensitive manner
    Galloway, SM
    McNatty, KP
    Cambridge, LM
    Laitinen, MPE
    Juengel, JL
    Jokiranta, TS
    McLaren, RJ
    Luiro, K
    Dodds, KG
    Montgomery, GW
    Beattie, AE
    Davis, GH
    Ritvos, O
    [J]. NATURE GENETICS, 2000, 25 (03) : 279 - 283
  • [5] Oocyte-secreted factors: regulators of cumulus cell function and oocyte quality
    Gilchrist, Robert B.
    Lane, Michelle
    Thompson, Jeremy G.
    [J]. HUMAN REPRODUCTION UPDATE, 2008, 14 (02) : 159 - 177
  • [6] Oocyte bone morphogenetic protein 15, but not growth differentiation factor 9, is increased during gonadotropin-induced follicular development in the immature mouse and is associated with cumulus oophorus expansion
    Gueripel, Xavier
    Brun, Veronique
    Gougeon, Alain
    [J]. BIOLOGY OF REPRODUCTION, 2006, 75 (06) : 836 - 843
  • [7] Quantitative analysis of bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9) gene expression in calf and adult bovine ovaries
    Hosoe, Misa
    Kaneyama, Kanako
    Ushizawa, Koichi
    Hayashi, Ken-go
    Takahashi, Toru
    [J]. REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY, 2011, 9
  • [8] Oocytes prevent cumulus cell apoptosis by maintaining a morphogenic paracrine gradient of bone morphogenetic proteins
    Hussein, TS
    Froiland, DA
    Amato, F
    Thompson, JG
    Gilchrist, RB
    [J]. JOURNAL OF CELL SCIENCE, 2005, 118 (22) : 5257 - 5268
  • [9] Krisher R.L., 2004, J. Anim. Sci., V82, P14, DOI [DOI 10.2527/2004.8213_SUPPLE14X, 10.2527/2004.8213_supplE14x]
  • [10] Differential gene expression of bone morphogenetic protein 15 and growth differentiation factor 9 during in vitro maturation of porcine oocytes and early embryos
    Li, Hou-Kuan
    Kuo, Ting-Yung
    Yang, Hsiu-Shan
    Chen, Lih-Ren
    Li, Steven Shoei-Lung
    Huang, Hurng-Wern
    [J]. ANIMAL REPRODUCTION SCIENCE, 2008, 103 (3-4) : 312 - 322
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