Novel Label-free Assay of Telomerase Based on DNAzyme

被引：2

作者：

Fan Hongliang ^{[1
,2
]}

Zhang Tao ^{[2
]}

Jin Wei ^{[2
]}

Jin Qinhan ^{[2
]}

机构：

[1] Zhejiang Acad Med Sci, Inst Hyg, Dept Environm Med, Hangzhou 310013, Zhejiang, Peoples R China

[2] Zhejiang Univ, Res Ctr Analyt Instrumentat, Inst Cyber Syst & Control, State Key Lab Ind Control Technol, Hangzhou 310058, Zhejiang, Peoples R China

来源：

CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE | 2015年 / 36卷 / 04期

基金：

中国国家自然科学基金;

关键词：

Telomerase; DNAzyme; Horseradish peroxidase; Signal amplification; Toehold-mediated strand displacement; DNA; AMPLIFICATION;

D O I：

10.7503/cjcu20141048

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A novel method for telomerase detection based on DNAzyme and toehold-mediated strand displacement was developed. Optimal designing of a hairpin probe ensured efficient inhibition of DNAzyme activity by preventing it from forming the G-quadruplex structure. After the substrate TS was elongated by telomerase, the original hairpin structure could be destroyed through toehold-mediated strand displacement, so that the free DNAzyme. was released, which catalyzed the oxidation of ABTS(2-) to generate an absorbance increase at 415 nm. The results indicated that the proposed method could detect the telomerase as low as 500 Hela cells equivalent. Besides, this method is simple and requires no fluorescence labeling or complicated surface modification, and thus is expected to have a wide use in routine telomerase analysis of tumor cells.

引用

页码：625 / 630

页数：6

共 18 条

[1] Telomeres and aging [J].