An histidine covalent receptor and butenolide complex mediates strigolactone perception

被引：212

作者：

de Saint Germain, Alexandre ^{[1
,2
,3
,4
,5
,6
]}

Clave, Guillaume ^{[7
,8
,9
]}

Badet-Denisot, Marie-Ange ^{[7
,8
,9
]}

Pillot, Jean-Paul ^{[1
,2
,3
,4
]}

Cornu, David ^{[10
,11
,12
]}

Le Caer, Jean-Pierre ^{[7
,8
,9
]}

Burger, Marco ^{[5
,6
]}

Pelissier, Frank ^{[7
,8
,9
]}

Retailleau, Pascal ^{[7
,8
,9
]}

Turnbull, Colin ^{[13
]}

Bonhomme, Sandrine ^{[1
,2
,3
,4
]}

Chory, Joanne ^{[5
,6
]}

Rameau, Catherine ^{[1
,2
,3
,4
]}

Boyer, Francois-Didier ^{[1
,2
,3
,4
,7
,8
,9
]}

机构：

[1] INRA, Inst Jean Pierre Bourgin, Versailles, France

[2] AgroParisTech, Inst Jean Pierre Bourgin, Versailles, France

[3] CNRS, Inst Jean Pierre Bourgin, Versailles, France

[4] Univ Paris Saclay, Inst Jean Pierre Bourgin, Versailles, France

[5] Salk Inst Biol Studies, Howard Hughes Med Inst, La Jolla, CA 92037 USA

[6] Salk Inst Biol Studies, Plant Biol Lab, La Jolla, CA 92037 USA

[7] CNRS UPR2301, Inst Chim Subst Nat, Gif Sur Yvette, France

[8] Univ Paris 11, Inst Chim Subst Nat, Gif Sur Yvette, France

[9] Univ Paris Saclay, Inst Chim Subst Nat, Gif Sur Yvette, France

[10] CNRS, Inst Biol Integrat Cellule, Gif Sur Yvette, France

[11] CEA, Inst Biol Integrat Cellule, Gif Sur Yvette, France

[12] Univ Paris Saclay, Inst Biol Integrat Cellule, Gif Sur Yvette, France

[13] Imperial Coll London, Div Cell & Mol Biol, London, England

来源：

NATURE CHEMICAL BIOLOGY | 2016年 / 12卷 / 10期

基金：

美国国家卫生研究院;

关键词：

ACTS DOWNSTREAM; ARABIDOPSIS; PROTEIN; RESPONSES; KARRIKIN; PEA; INHIBITION; DWARF14; GENES; HORMONES;

D O I：

10.1038/nchembio.2147

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Strigolactone plant hormones control plant architecture and are key players in both symbiotic and parasitic interactions. They contain an ABC tricyclic lactone connected to a butenolide group, the D ring. The DWARF14 (D14) strigolactone receptor belongs to the superfamily of alpha/beta-hydrolases, and is known to hydrolyze the bond between the ABC lactone and the D ring. Here we characterized the binding and catalytic functions of RAMOSUS3 (RMS3), the pea (Pisum sativum) ortholog of rice (Oryza sativa) D14 strigolactone receptor. Using new profluorescent probes with strigolactone-like bioactivity, we found that RMS3 acts as a single-turnover enzyme that explains its apparent low enzymatic rate. We demonstrated the formation of a covalent RMS3-D-ring complex, essential for bioactivity, in which the D ring was attached to histidine 247 of the catalytic triad. These results reveal an undescribed mechanism of plant hormone reception in which the receptor performs an irreversible enzymatic reaction to generate its own ligand.

引用

页码：787 / +

页数：13

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