Evaluation of the antioxidant activity of five endemic Ligustrum species leaves from Taiwan flora in vitro

被引:52
作者
Wu, Chi-Rei [1 ]
Lin, Wen-Hsin [2 ]
Hseu, You-Cheng [3 ]
Lien, Jin-Cherng [4 ]
Lin, Yung-Ta [5 ]
Kuo, Tzu-Ping [1 ]
Ching, Hui [6 ]
机构
[1] China Med Univ, Coll Pharm, Grad Inst Chinese Pharmaceut Sci, Taichung 40402, Taiwan
[2] China Med Univ, Coll Pharm, Dept Pharm, Taichung 40402, Taiwan
[3] China Med Univ, Coll Pharm, Dept Cosmeceut, Taichung 40402, Taiwan
[4] China Med Univ, Coll Pharm, Grad Inst Pharmaceut Chem, Taichung 40402, Taiwan
[5] Taichung Tzu Chi Gen Hosp, Dept Pharm, Tanzih Township 427, Taichung County, Taiwan
[6] Taichung Hosp, Dept Hlth, Taichung 40403, Taiwan
关键词
Ligustrum; Antioxidant activity; Phenolics; Phenylpropanoids; DPPH method; Reactive oxygen species (ROS); Lipid peroxidation; RADICAL SCAVENGING ACTIVITY; OXIDATIVE STRESS; NEURODEGENERATIVE DISEASES; PHENYLETHANOID GLYCOSIDES; PHENOLIC-COMPOUNDS; MEDICINAL-PLANTS; ROBUSTUM; CONSTITUENTS; PURPURASCENS; EXTRACTS;
D O I
10.1016/j.foodchem.2011.01.041
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Leaves from the plant species belonging to the genus Ligustrum are widely used as tea or herbal medicine in Europe, China, and Japan. The antioxidant properties of five Ligustrum species from Taiwan were compared using in vitro antioxidant methods such as DPPH radical scavenging, TEAC, and FRAP assays. Cell-based antioxidant methods were used, including Fe2+/ascorbate-induced lipid peroxidation on brain homogenate and AAPH-induced erythrocyte haemolysis. The amounts of major phenolic compounds from the Ligustrum species, including phenylpropanoids, flavonoids, and iridoids, were determined by spectrophotometric methods. The results showed that all Ligustrum species exhibited antioxidant, radical-scavenging, anti-haemolytic, and lipid peroxidation-inhibiting activities at different magnitudes of potency. A significant correlation was found between antioxidant activity and the amount of antioxidant components, in particular, total phenolics and phenylpropanoids. Among all Ligustrum species from Taiwan, Ligustrum morrisonense is presented as potential source of natural antioxidants. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码:564 / 571
页数:8
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