Isolation and characterization of Getah virus from pigs in Guangdong province of China

被引:42
作者
Xing, Chaonan [1 ,2 ]
Jiang, Jianfeng [2 ,3 ]
Lu, Zongji [3 ]
Mi, Shijiang [2 ,4 ]
He, Biao [2 ]
Tu, Changchun [1 ,2 ]
Liu, Xiufan [1 ]
Gong, Wenjie [2 ,4 ]
机构
[1] Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Coll Vet Med, Yangzhou, Jiangsu, Peoples R China
[2] Acad Mil Med Sci, Acad Mil Med Sci, Inst Mil Vet Med, Changchun, Peoples R China
[3] Foshan Univ, Sch Life Sci & Engn, Foshan, Peoples R China
[4] Jilin Univ, Coll Vet Med, Minist Educ, Key Lab Zoonoses Res, Changchun, Peoples R China
基金
中国国家自然科学基金;
关键词
cytopathic effect; genome analysis; GETV; pig; ALPHAVIRUS;
D O I
10.1111/tbed.13567
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Getah virus (GETV) is a mosquito-borne virus that is widely distributed in Asian countries including China, in which the first case of equine GETV infection was reported in Guangdong province in August 2018. In this study, GETVs were detected in two classical swine fever virus-positive samples collected from swine herds in Foshan city, Guangdong province, 2018. Infection of porcine PK-15 cells produced rapid cytopathic effects (CPEs), including shrinking, rounding and detaching, and peak titre of 10(9.3) TCID50/ml occurred at 24 hr post-infection. Electron microscopy and ultra-thin sectioning revealed spherical GETV particles of 70 nm diameter with an isometric interior and are found to be lining the outer membrane of infected cells. Whole-genome analysis showed that the two GETV isolates are identical to each other and cluster with Group III strains of GETV, sharing 96.1%-99.7% nucleotide sequence identity with all available reference strains. The most closely relative of the obtained GETV isolates was porcine strain HNJZ-S2 from Henan province (99.7%), with 98.6% sequence identity shared with equine GETV strain GZ201808 first identified in Guangdong province, indicating different sources for porcine and equine GETV infections in this region. No evidence of GETV infection was found in 497 archived porcine samples collected between 1990 and 2018 in Guangdong province.
引用
收藏
页码:2249 / 2253
页数:5
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