Preparation and characterization of a specific monoclonal antibody against CIAPIN1

被引：19

作者：

Hao, ZM ^{[1
]}

Qiao, TD ^{[1
]}

Jin, XH ^{[1
]}

Li, XH ^{[1
]}

Gao, J ^{[1
]}

Fan, DM ^{[1
]}

机构：

[1] Fourth Mil Med Univ, Xijing Hosp, Inst Digest Dis, Dept Gastroenterol,State Key Lab Canc Biol, Xian 710032, Peoples R China

来源：

HYBRIDOMA | 2005年 / 24卷 / 03期

关键词：

D O I：

10.1089/hyb.2005.24.141

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cytokine-induced antiapoptosis inhibitor 1 (CIAPIN1) is a newly identified antiapoptosis molecule and a mediator of gastric MDR. We cloned cDNA of human CIAPIN1 by RT-PCR and constructed prokaryotic expression vectors of human CIAPIN1 by inserting human CIAPIN1 coding region into pET28-a(+) and pGEX-4T-1, respectively. The fusion proteins were expressed in Escherichia coli and purified by affinity chromotography. Monoclonal antibody (MAb) against CIAPIN1 was obtained with standard cell fusion technique and ELISA screening. Immunohistochemistry and Western blot showed that the anti-CIAPIN1 MAb recognizes human and mouse CIAPIN1 protein in both native and denatured form. Western blotting confirmed that the expression of CIAPIN1 was upregulated in MDR gastric cancer cell lines. This MAb will be a useful tool for the detection of CIAPIN1 protein in future studies.

引用

页码：141 / 145

页数：5

共 11 条

[1]

DESTFASEKS G, 1980, J IMMUNOL METHODS, V35, P1

[2] UNDER PROPER EXPERIMENTAL CONDITIONS THE SOLID-PHASE ANTIGEN DOES NOT DISRUPT THE LIQUID-PHASE EQUILIBRIUM WHEN MEASURING DISSOCIATION-CONSTANTS BY COMPETITION ELISA [J].

FRIGUET, B ;

CHAFFOTTE, AF ;

DJAVADIOHANIANCE, L ;

GOLDBERG, ME .

JOURNAL OF IMMUNOLOGICAL METHODS, 1995, 182 (01) :145-147

[3] MEASUREMENTS OF THE TRUE AFFINITY CONSTANT IN SOLUTION OF ANTIGEN-ANTIBODY COMPLEXES BY ENZYME-LINKED IMMUNOSORBENT-ASSAY [J].

FRIGUET, B ;

CHAFFOTTE, AF ;

DJAVADIOHANIANCE, L ;

GOLDBERG, ME .

JOURNAL OF IMMUNOLOGICAL METHODS, 1985, 77 (02) :305-319

[4] DERIVATION OF SPECIFIC ANTIBODY-PRODUCING TISSUE-CULTURE AND TUMOR LINES BY CELL-FUSION [J].

KOHLER, G ;

MILSTEIN, C .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1976, 6 (07) :511-519

[5] Genome duplications and other features in 12 Mb of DNA sequence from human chromosome 16p and 16q [J].

Loftus, BJ ;

Kim, UJ ;

Sneddon, VP ;

Kalush, F ;

Brandon, R ;

Fuhrmann, J ;

Mason, T ;

Crosby, ML ;

Barnstead, M ;

Cronin, L ;

Mays, AD ;

Cao, YC ;

Xu, RX ;

Kang, HL ;

Mitchell, S ;

Eichler, EE ;

Harris, PC ;

Venter, JC ;

Adams, MD .

GENOMICS, 1999, 60 (03) :295-308

[6] AFFINITY PURIFICATION OF HISTIDINE-TAGGED PROTEINS [J].

SCHMITT, J ;

HESS, H ;

STUNNENBERG, HG .

MOLECULAR BIOLOGY REPORTS, 1993, 18 (03) :223-230

[7] Identification of a cytokine-induced antiapoptotic molecule anamorsin essential for definitive hematopoiesis [J].

Shibayama, H ;

Takai, E ;

Matsumura, I ;

Kouno, M ;

Morii, E ;

Kitamura, Y ;

Takeda, J ;

Kanakura, Y .

JOURNAL OF EXPERIMENTAL MEDICINE, 2004, 199 (04) :581-592

[8] SINGLE-STEP PURIFICATION OF POLYPEPTIDES EXPRESSED IN ESCHERICHIA-COLI AS FUSIONS WITH GLUTATHIONE S-TRANSFERASE [J].

SMITH, DB ;

JOHNSON, KS .

GENE, 1988, 67 (01) :31-40

[9] Intractable cancers: The many faces of multidrug resistance and the many targets it presents for therapeutic attack [J].

Stein, WD ;

Bates, SE ;

Fojo, T .

CURRENT DRUG TARGETS, 2004, 5 (04) :333-346

[10] Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences [J].

Strausberg, RL ;

Feingold, EA ;

Grouse, LH ;

Derge, JG ;

Klausner, RD ;

Collins, FS ;

Wagner, L ;

Shenmen, CM ;

Schuler, GD ;

Altschul, SF ;

Zeeberg, B ;

Buetow, KH ;

Schaefer, CF ;

Bhat, NK ;

Hopkins, RF ;

Jordan, H ;

Moore, T ;

Max, SI ;

Wang, J ;

Hsieh, F ;

Diatchenko, L ;

Marusina, K ;

Farmer, AA ;

Rubin, GM ;

Hong, L ;

Stapleton, M ;

Soares, MB ;

Bonaldo, MF ;

Casavant, TL ;

Scheetz, TE ;

Brownstein, MJ ;

Usdin, TB ;

Toshiyuki, S ;

Carninci, P ;

Prange, C ;

Raha, SS ;

Loquellano, NA ;

Peters, GJ ;

Abramson, RD ;

Mullahy, SJ ;

Bosak, SA ;

McEwan, PJ ;

McKernan, KJ ;

Malek, JA ;

Gunaratne, PH ;

Richards, S ;

Worley, KC ;

Hale, S ;

Garcia, AM ;

Gay, LJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (26) :16899-16903

← 1 2 →