Conserved MicroRNAs in Chinese Hamster Ovary Cell Lines

被引：34
作者：
Johnson, Kathryn C. [1 ]
Jacob, Nitya M. [1 ]
Nissom, Peter Morin [2 ]
Hackl, Matthias [1 ]
Lee, Lim Hseuh [2 ]
Yap, Miranda [2 ]
Hu, Wei-Shou [1 ]
机构：
[1] Univ Minnesota, Dept Chem Engn & Mat Sci, Minneapolis, MN 55455 USA
[2] Agcy Sci Technol & Res, Bioproc Technol Inst, Singapore, Singapore
来源：
BIOTECHNOLOGY AND BIOENGINEERING | 2011年 / 108卷 / 02期
关键词：
Chinese hamster; CHO cells; microRNA; miRNA; Illumina sequencing; MONOCLONAL-ANTIBODY; EXPRESSION; DATABASE; GENES; IDENTIFICATION; BIOGENESIS; COMPONENT; RESOURCE; SEQUENCE;
D O I：
10.1002/bit.22940
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
MicroRNAs (miRNAs), a class of short (20-24 nt) non-coding RNAs that direct post-transcriptional repression of messenger RNAs, increasingly have been shown to play a key role in regulating cellular physiology. We investigated the prevalence of miRNAs in Chinese hamster ovary (CHO) cells by high-throughput sequencing. Six cDNA libraries of small RNAs from four CHO cell lines were constructed and sequenced by Illumina sequencing. Three hundred fifty distinct miRNA and miRNA* sequences were identified through homology with other species, including mouse, rat, and human. While the majority of the identified miRNAs appear to be expressed ubiquitously, many miRNAs were found to have a wide range of expression levels between cell lines. The identification of these miRNAs will facilitate investigations of their contribution to the hyperproductivity trait. Biotechnol. Bioeng. 2011; 108: 475-480. (C) 2010 Wiley Periodicals, Inc.
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收藏
页码：475 / 480
页数：6
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