Identification of novel immunogenic proteins in pathogenic Haemophilus parasuis based on genome sequence analysis

被引：25

作者：

Hong, Minhee ^{[1
]}

Ahn, Jungoh ^{[1
]}

Yoo, Sungsik ^{[2
]}

Hong, Jiyeon ^{[1
]}

Lee, Eungyu ^{[1
]}

Yoon, Injoong ^{[2
]}

Jung, Joon-ki ^{[1
]}

Lee, Hongweon ^{[1
]}

机构：

[1] KRIBB, Ctr Biotechnol Proc Engn, Taejon 305600, South Korea

[2] Choong Ang Vaccine Lab, Taejon 305348, South Korea

来源：

VETERINARY MICROBIOLOGY | 2011年 / 148卷 / 01期

关键词：

Haemophilus parasuis; Reverse vaccinology; Antigenic protein; OUTER-MEMBRANE PROTEINS; VIRULENCE; IRON;

D O I：

10.1016/j.vetmic.2010.07.023

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Haemophilus parasuis causes contagious porcine Glasser's disease, which is occurring worldwide and leads to severe losses in the pig industry. To identify novel antigen candidates against this disease, 22 surface-exposed or secreted proteins were selected from the annotated H. parasuis genome by reverse vaccinology strategy. Expression of these proteins in Escherichia coli was attempted. Immunogenicity of the expressed candidates was assessed using Western blot analysis with mouse-derived antiserum prepared with whole bacteria of H. parasuis serovar 4 or 5. Three ABC-type transporters (OppA, YfeA and PlpA) and 1 curli protein assembly (CsgG) were identified as potent immunogenic proteins. The proteins show cross-reactions when tested with sera raised against serovars 4 and 5 of H. parasuis. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：89 / 92

页数：4

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